C4840 Methods for labelling and immobilization of biomolecules

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 13:00–14:50 C05/114
Prerequisites
basic knowledge of biochemistry and chemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Abstract
Students will gain theoretical knewledge about modification of biomolecules based on conjugation reactions, introduction of labels and immobilization on surface of polymers and sensing surfaces. They will obtain overview of reagents for conjugation and labelling (enzymes, fluorophores, biotinylation, liposomes, nanoparticles).
Key topics
  • 1. Modification of proteins and peptides, side chains of amino acids, prosthetic groups.
  • 2. Modification of nucleotides, DNA, RNA. Modification of (poly)saccharides, glycoconjugates.
  • 3. Insertion of additional reactive groups into biomolecules. Basic chemical reactions for conjugation.
  • 4. Bioconjugations, homo/heterobifunctional reagents. Cleavable links.
  • 5. Fluorescent labels, fluoresceins, rhodamins, coumarins, FRET.
  • 6. Biotinylation, chelates, oligohistidine tags, boronate complexes. Radiolabels, nanoparticles, liposomes.
  • 7. Enzyme labels.
  • 8. Immobilization of biomolecules for affinity chromatography.
  • 9. Activation of inert sensing surfaces - gold, platinum, graphite.
  • 10. Characterization of bioconjugates.
  • 11. Hapten-carrier conjugations.
  • 12. Immunosensors, sensing microarrays and DNA biochips.
Study resources and literature
    recommended literature
  • HERMANSON, Greg T. Bioconjugate techniques. San Diego: Academic Press, 1995, xxv, 785 s. ISBN 0-12-342336-8. info
Approaches, practices, and methods used in teaching
lecture course
Method of verifying learning outcomes and course completion requirements
oral examination, random selection of 3 questions - solution of model problems, preparation of answers, discussion with the teacher. Min. 50% of correct answers.
Language of instruction
Czech
Further Comments
Study Materials
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025, Spring 2026, Spring 2027.
  • Enrolment Statistics (Spring 2014, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2014/C4840