C4840 Methods for labelling and immobilization of biomolecules

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Tue 13:00–14:50 C05/114
Prerequisites
basic knowledge of biochemistry and chemistry
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
Students will gain theoretical knewledge about modification of biomolecules based on conjugation reactions, introduction of labels and immobilization on surface of polymers and sensing surfaces. They will obtain overview of reagents for conjugation and labelling (enzymes, fluorophores, biotinylation, liposomes, nanoparticles).
Learning outcomes
Students will learn methods for conjugation of biomolecules with labels as enzymes, fluorophores and nanoparticles. They will manage principles of immobilization techniques for preparation of affinity supports and surfaces for purification and bioanalytical applications.
Syllabus
  • 1. Modification of proteins and peptides, side chains of amino acids, prosthetic groups.
  • 2. Modification of nucleotides, DNA, RNA. Modification of (poly)saccharides, glycoconjugates.
  • 3. Insertion of additional reactive groups into biomolecules. Basic chemical reactions for conjugation.
  • 4. Bioconjugations, homo/heterobifunctional reagents. Cleavable links.
  • 5. Fluorescent labels, fluoresceins, rhodamins, coumarins, FRET.
  • 6. Biotinylation, chelates, oligohistidine tags, boronate complexes. Radiolabels, nanoparticles, liposomes.
  • 7. Enzyme labels.
  • 8. Immobilization of biomolecules for affinity chromatography.
  • 9. Activation of inert sensing surfaces - gold, platinum, graphite.
  • 10. Characterization of bioconjugates.
  • 11. Hapten-carrier conjugations.
  • 12. Immunosensors, sensing microarrays and DNA biochips.
Literature
    recommended literature
  • HERMANSON, Greg T. Bioconjugate techniques. San Diego: Academic Press, 1995, xxv, 785 s. ISBN 0-12-342336-8. info
Teaching methods
lecture course
Assessment methods
oral examination, random selection of 3 questions - solution of model problems, preparation of answers, discussion with the teacher. Min. 50% of correct answers.
Language of instruction
Czech
Further Comments
Study Materials
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2019, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2019/C4840