Bi2120c Cytology, histology, embryology practical training

Faculty of Science
Spring 2019
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Irena Lauschová, Ph.D. (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
Mgr. Hana Kotasová, Ph.D. (seminar tutor)
MUDr. Veronika Sedláková, Ph.D. (seminar tutor)
doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Vaňhara, Ph.D.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Contact Person: doc. RNDr. Miroslav Králík, Ph.D.
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
Bi2120c/01: Mon 18. 2. to Fri 17. 5. Tue 7:00–9:30 F01B1/122
Bi2120c/02: Mon 18. 2. to Fri 17. 5. Wed 9:00–11:30 F01B1/122
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue, composition of blood, basic principles of hematopoiesis.
3. Identify tissue types in histologic slides.
4. Describe gamete development, explain principle of meiosis and regulation of gametogenesis, describe ovarian and menstrual cycles, their relationship and endocrine regulation.
5. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord in detail. Characterize stages of embryonic development.
6. Characterize the course of pregnancy and delivery and potential anomalies, describe changes in number of fetuses and respective alterations in placenta and fetal membranes.
Syllabus
  • Introduction, organization of practicals, teaching aids. Overview of histological methods. Tissue processing for light microscopy - explanation and film. Basic histochemistry and immunocytochemistry.
  • Cytology: Ultrastructure of the cell nucleus (during interphase and mitosis). Ultrastructure of cell organelles (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes, peroxisomes, and the centriol). Cell inclusions. The arrangement of cell surfaces: apical, lateral, and basal ones. Intercellular junctions: adhering, occluding, and communicating ones.
  • Blood elements: Preparation of blood smears and their staining. Cytology of formed elements of blood. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Slide: A smear of peripheral blood stained by Pappenheim's method.
  • Hematopoiesis: Erythropoiesis, granulopoiesis and development of thrombocytes. Development of lymphocytes and monocytes. Microscopic structure of the bone marrow. Slide: A smear of bone marrow stained by Pappenheim's method.
  • Embryology I. Interactive repetition of early human development. Outline of the development of fetal membranes: amnion and chorion. Placenta, types of placenta, development of placenta in humans. Structure and function of human placenta. Development of umbilical cord. Anomalies of placenta and umbilical cord. Uterus and pregnancy. Development of external form of the embryo.
  • Embryology II. Interactive repetition of Embryology I. Parturition. FMarks of full-term and mature fetus. The rule of Haase. Position, posture, habitus and presentation of the fetus. Multiple pregnancy: mono- and dizygotic twins, arrangements of fetal membranes. Prenatal diagnostic and medicine. Essentials of teratology. Critical periods.
  • General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin-eosine-safron /HES/ (oesophagus), AZAN (prostate), impregnation (cerebellum).
  • Connective tissue proper - classification and examples. Slides: Funiculus umbilicalis, Oesophagus, Posterior segment of the eye, Lien, Aorta.
  • Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, aAricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification.
  • Epithelial tissue - epithelial membranes, glandular epithelium. (covering epithelia). Slides: Ren, Vesica fellea, Trachea, Oesophagus, Urether, Palpebra, Intestinum tenue, Glandula parotis, Glandula submandibularis.
  • Muscle tissue. Slides: Apex linguae, Intestinum crassum, Myocardium.
  • Nerve tissue. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
Literature
  • ČECH, Svatopluk, Drahomír HORKÝ, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a metody vyšetřování tkání a orgánů (Histological practicals and methods of investigation of tissues and organs). 1.dotisk 1. vydání. Brno: Vydavatelství MU Brno-Kraví hora, 2002, 162 pp. ISBN 80-210-1774-0. info
  • ČECH, Svatopluk, Drahomír HORKÝ, Ladislav ILKOVICS, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a laboratorní technika. Učební text a atlas (Histological practicals and technique. Textbook and Atlas). 1. vydání. Brno: Multimediální text podpořený FRVŠ č. projektu 357/2006 (F3/d), 2006. URL info
Teaching methods
Content of this course includes study of slides and electron micrographs in microscopic hall as well as class discussion on the base of materials in the text book.
Assessment methods
A precondition for obtaining the course-unit credit is the full attendance and demonstration of the basic knowledge in the form of a test or oral examination.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2019, recent)
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