C1680 Laboratory Course in Bioanalytical chemistry

Faculty of Science
Spring 2026
Extent and Intensity
0/0/3. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Dr. rer. nat. habil. Hans-Heiner Gorris (seminar tutor)
Guaranteed by
Dr. rer. nat. habil. Hans-Heiner Gorris
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites (in Czech)
C1610 General and Inorg. Chem.-sem. && NOW( C1670 Bioanalytical chemistry )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main goals of the subject are practicing topics discussed in course C1670 Bioanalytical chemistry: spectroscopic, chromatographic, electromigration, and physical methods of biochemical analysis.
Learning outcomes
At the end of this course, the students will be able to theoretically understand and practically apply the spectroscopic, chromatographic, electromigration, and physical methods of analysis in biochemistry. They will able to apply this knowledge in their scientific research, working on bachelor, master, and PhD theses and finally also during their following employment.
Syllabus
1. Electronic spectra of molecules, transitions, ground and excited state, influence of the environment, UV-Vis spectroscopy, use to determine substances and study the structure of proteins.
2. Chiroptical methods and their principle. ORD and CD and their use to study protein conformation.
3. IR spectroscopy and its use to study protein structure, Raman scattering and its use to study protein structure.
4. Atomic absorption and atomic emission spectroscopy.
5. Luminescence methods. Quantum yield, influence of the environment, use to determine substances and study protein conformation, fluorescence quenching, energy transfer.
6. Microscopy. Methods of optical microscopy, electron microscopy, and scanning probe microscopy.
7. Immunochemical methods. Preparation and use of antibodies in bioanalytical chemistry, formats of immunochemical assays.
8. Biosensors. Biocatalytic and bioaffinity sensors, the most important types of transducers.
9. Chromatographic methods, principles (adsorption, ion-exchange), chromatofocusing.
10. Electromigration methods, principles, zone electrophoresis, IEF, isotachophoresis, blotting.
11. Mass spectrometry.
Literature
    recommended literature
  • HOLTZHAUER, Martin. Basic methods for the biochemical lab. 1st english ed. Berlin: Springer, 2006, xviii, 251. ISBN 3540327851. info
  • PINGOUD, Alfred. Biochemical methods : a concise guide for students and researchers. Weinheim: Wiley-VCH, 2002, xiii, 360. ISBN 3527302999. info
Teaching methods
Seminar focused on practicing topics discussed in course C1670 Bioanalytical chemistry.
Assessment methods
Written test.
Language of instruction
English
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught every other week.
General note: Vyučují učitelé katedry analytické chemie.
  • Permalink: https://is.muni.cz/course/sci/spring2026/C1680