C4230 Biochemistry - laboratory course

Faculty of Science
Spring 2026
Extent and Intensity
0/0/6. 6 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. Lukáš Faltinek, Ph.D. (seminar tutor)
Mgr. Jiří Kučera, Ph.D. (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Lukáš Faltinek, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C3181 Biochemistry I && ( C4221 Introductory Biochemistry Laboratory || C1100 Introductory Chemistry Lab )
Completion of the course Biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the laboratory course is to study the basic properties of compounds involved in biochemical processes and methodological approaches to the study and analytical use of enzymes. The first part covers the qualitative and quantitative determination of carbohydrates, amino acids, proteins and nucleic acids. The second part deals with enzyme kinetics and further study of enzyme properties.
Learning outcomes
The students will be able to: - perform basic laboratory operations in a biochemical laboratory - operate common laboratory instruments used in a biochemical laboratory - mathematically evaluate measured data - interpret simple results of their work
Syllabus
  • 01. Work safety rules. Organization of laboratory exercises. Preparation of dilute solution, pipetting, and photometry.
  • 02. Isolation, analysis, and determination of deoxyribonucleic acid (DNA).
  • 03. Qualitative and quantitative determination of amino acids.
  • 04. Quantitative determination of proteins.
  • 05. Qualitative and quantitative determination of carbohydrates.
  • 06. Isolation and analysis of lipids.
  • 07. Sucrase - immobilization, substrate specificity, activity determination.
  • 08. pH optimum of enzyme reaction.
  • 09. Enzyme kinetics.
  • 10. Analytical use of proteins.
  • 11. Biological electron transfer chains.
Literature
    recommended literature
  • VOET, Donald; Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999, 1 sv. ISBN 0471586501. info
Teaching methods
Laboratory course
Assessment methods
The first laboratory exercise is dedicated to the teaching of safety in the biochemistry laboratory. Students also write an entrance test, the successful completion of which is a condition for participation in the exercise. Before each subsequent exercise, a written test is given to check the student's preparation for the task. In all tests, including the placement test, the student must answer at least 65 % of the questions correctly.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught each semester.
The course is taught every week.
General note: Vyučují učitelé katedry biochemie.
Information on course enrolment limitations: V podzimním semestru se vypisuje pouze jedna skupina pro 12 studentů chemických oborů jako náhrada za JS
The course is also listed under the following terms Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2026, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2026/C4230