PřF:C8160 Enzymology - Course Information

C8160 Enzymology

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Igor Kučera, DrSc. (lecturer)

Guaranteed by

prof. RNDr. Igor Kučera, DrSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science

Timetable

Tue 8:00–9:50 C05/114

Prerequisites

C4182 Biochemistry II || C3580 Biochemistry
Basic courses in biochemistry, organic chemistry and physical chemistry are recommended.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Molecular Biology and Genetics (programme PřF, B-EXB)
• Molecular Biology and Genetics (programme PřF, N-EXB)

Course objectives

The course aims at providing detailed background information on the most important aspects of enzymatic reactions including structure of enzymes, enzyme activity measurement, enzyme purification, reaction mechanisms, kinetics, and bioanalytical and industrial applications of biocatalysis.

Learning outcomes

The student will get a basic idea about the properties of enzymes and the relationship between their structure and mechanisms of catalytic action. He/she will know the most important representatives of individual classes of enzymes, their use in practice and the most common methods for the determination of enzyme aktivity and substrate concentration. He/she also will be able to process data from kinetic studies and interpret the results in relation to the reaction mechanism.

Syllabus

• 1) First sight into the world of enzymes: structure, specifity, nomenclature. 2) Enzyme activity and their measurement. 3) Isolation of enzymes from the natural sources and expression systems. 4) Enzyme catalysis from the point of view of organic chemistry. 5) Enzyme catalysis from the point of view of physical chemistry. 6) Basic theoretical concepts in enzyme kinetics. 7) Effects of environmental factors (pH, temperature) on enzymes. 8) Reversible and irreversible inhibition of enzymes. 9) Kinetics of multisubstrate enzyme reactions. 10) Cooperativity in enzyme function. 11) Immobilized enzyme systems. 12) Enzymes as tools in bioanalysis. 13) Use of enzymes in technology and medicine. Enzyme engineering.

Literature

recommended literature
• MACHOLÁN, Lumír. Enzymologie. 2. upr. vyd. Brno: Vydavatelství Masarykovy univerzity, 1994, 152 s. ISBN 8021010398. info
• KOTYK, Arnošt and Jaroslav HORÁK. Enzymová kinetika. Vyd. 1. Praha: Academia, 1977, 268 s. info
• PRICE, Nicholas C. and Lewis STEVENS. Fundamentals of enzymology : the cell and molecular biology of catalytic proteins. 3rd ed. Oxford: Oxford University Press, 1999, xviii, 478. ISBN 019850229X. info
• BISSWANGER, Hans. Practical enzymology. 2nd, completely rev. ed. Weinheim: Wiley-Blackwell, 2011, xvi, 360. ISBN 9783527320769. info
• LESKOVAC, Vladimir. Comprehensive enzyme kinetics. New York: Kluwer Academic/Plenum publishers, 2003, xi, 438. ISBN 0306467127. info
• CORNISH-BOWDEN, Athel. Fundamentals of enzyme kinetics. Rev. ed. London: Portland Press, 1995, xiii, 343. ISBN 1855780720. info

Teaching methods

Lectures

Assessment methods

A one semester lecture course, 2 x 45 min per week. The examination is mainly written. It takes 60 min and consists of three parts, one of them being concerned with quantitative aspects. An oral part of the examination then follows.

Language of instruction

Czech

Further Comments

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• C8170 Enzymology - seminar
  NOW(C8160)  

• Enrolment Statistics (Autumn 2022, recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2022/C8160