Apolipoprotein E gene polymorphisms in chronic periodontitis

a 2013

Apolipoprotein E gene polymorphisms in chronic periodontitis

BOŘILOVÁ LINHARTOVÁ, Petra; Jan MÁCHAL; Jiřina BÁRTOVÁ; Hana POSKEROVÁ; Jan VOKURKA et al.

Základní údaje

Originální název

Apolipoprotein E gene polymorphisms in chronic periodontitis

Autoři

BOŘILOVÁ LINHARTOVÁ, Petra; Jan MÁCHAL; Jiřina BÁRTOVÁ; Hana POSKEROVÁ; Jan VOKURKA; Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ

Vydání

FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457. 2013

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/13:00065618

Organizační jednotka

Lékařská fakulta

ISSN

UT WoS

000325919202433

Klíčová slova anglicky

ApoE; chronic periodontitis; gene polymorphism; microbial colonization

Změněno: 26. 7. 2013 13:40, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Anotace

V originále

Objective: Periodontal diseases can be related to common systemic conditions, and, as recently described, alterations in blood lipid levels. The aim of this study was to analyze two apolipoprotein E (ApoE) gene polymorphisms in patients with chronic periodontitis (CP) in relation to periodontopathic bacteria and lipid levels. Methods: 469 unrelated subjects were included in this case-control study. Genomic DNA of 294 patients with CP and 175 healthy/non-periodontitis controls were genotyped, using the real-time polymerase chain reaction (RT-PCR) method monitored by SYBR Green, for ApoE (rs429358 and rs7412) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit and lipid levels were measured in a subgroup of subjects. Results: There was no evidence for a significant association between ApoE gene polymorphisms and CP (E2/E2 vs. E3/E3: OR=0.63, 95%CI=0.12-3.15; E2/E3 vs. E3/E3: OR=1.61, 95%CI=0.84-3.11; E2/E4 vs. E3/E3: OR=1.25, 95%CI=0.23-6.95; E3/E4 vs. E3/E3: OR=1.11, 95%CI=0.65-1.88; E4/E4 vs. E3/E3: OR=0.63, 95%CI=0.15-2.55; allele E2 vs. allele E3: OR=1.26, 95%CI=0.74-2.14; allele E4 vs. allele E3: OR=0.97, 95%CI=0.63-1.51). Patients with CP had increased total cholesterol and LDL levels compared to controls (P<0.05), however no significant differences were found for triglyceride and HDL levels. Gene variability in the ApoE gene influenced LDL levels marginally (P=0.07) but did not modify total cholesterol, triglyceride and HDL levels or the occurrence of periodontal pathogens in subgingival pockets. Conclusions: In Czech population studied, ApoE genetic variations rs429358 and rs7412 were not associated with susceptibility to and severity of chronic periodontitis or with the presence of periodontopathic bacteria.

Návaznosti

MUNI/A/0888/2012, interní kód MU

Název: Výzkum diagnostických a terapeutických přístupů v predikci onemocnění v orofaciální oblasti

Investor: Masarykova univerzita, Výzkum diagnostických a terapeutických přístupů v predikci onemocnění v orofaciální oblasti, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

NT11405, projekt VaV

Název: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Investor: Ministerstvo zdravotnictví ČR, Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Citovat

BOŘILOVÁ LINHARTOVÁ, Petra; Jan MÁCHAL; Jiřina BÁRTOVÁ; Hana POSKEROVÁ; Jan VOKURKA; Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Apolipoprotein E gene polymorphisms in chronic periodontitis. In FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457. 2013. ISSN 1742-4658.

@proceedings{1116975,
   author = {Bořilová Linhartová, Petra and Máchal, Jan and Bártová, Jiřina and Poskerová, Hana and Vokurka, Jan and Fassmann, Antonín and Izakovičová Hollá, Lydie},
   booktitle = {FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457.},
   keywords = {ApoE; chronic periodontitis; gene polymorphism; microbial colonization},
   language = {eng},
   title = {Apolipoprotein E gene polymorphisms in chronic periodontitis},
   year = {2013}
}

TY  - CONF
ID  - 1116975
AU  - Bořilová Linhartová, Petra - Máchal, Jan - Bártová, Jiřina - Poskerová, Hana - Vokurka, Jan - Fassmann, Antonín - Izakovičová Hollá, Lydie
PY  - 2013
TI  - Apolipoprotein E gene polymorphisms in chronic periodontitis
KW  - ApoE
KW  - chronic periodontitis
KW  - gene polymorphism
KW  - microbial colonization
N2  - Objective: Periodontal diseases can be related to common systemic conditions, and, as recently described, alterations in blood lipid levels. The aim of this study was to analyze two apolipoprotein E (ApoE) gene polymorphisms in patients with chronic periodontitis (CP) in relation to periodontopathic bacteria and lipid levels. Methods: 469 unrelated subjects were included in this case-control study. Genomic DNA of 294 patients with CP and 175 healthy/non-periodontitis controls were genotyped, using the real-time polymerase chain reaction (RT-PCR) method monitored by SYBR Green, for ApoE (rs429358 and rs7412) gene polymorphisms. Subgingival bacterial colonization was investigated by the DNA-microarray based on a periodontal pathogen detection kit and lipid levels were measured in a subgroup of subjects. Results: There was no evidence for a significant association between ApoE gene polymorphisms and CP (E2/E2 vs. E3/E3: OR=0.63, 95%CI=0.12-3.15; E2/E3 vs. E3/E3: OR=1.61, 95%CI=0.84-3.11; E2/E4 vs. E3/E3: OR=1.25, 95%CI=0.23-6.95; E3/E4 vs. E3/E3: OR=1.11, 95%CI=0.65-1.88; E4/E4 vs. E3/E3: OR=0.63, 95%CI=0.15-2.55; allele E2 vs. allele E3: OR=1.26, 95%CI=0.74-2.14; allele E4 vs. allele E3: OR=0.97, 95%CI=0.63-1.51). Patients with CP had increased total cholesterol and LDL levels compared to controls (P<0.05), however no significant differences were found for triglyceride and HDL levels. Gene variability in the ApoE gene influenced LDL levels marginally (P=0.07) but did not modify total cholesterol, triglyceride and HDL levels or the occurrence of periodontal pathogens in subgingival pockets. Conclusions: In Czech population studied, ApoE genetic variations rs429358 and rs7412 were not associated with susceptibility to and severity of chronic periodontitis or with the presence of periodontopathic bacteria.
ER  -

BOŘILOVÁ LINHARTOVÁ, Petra; Jan MÁCHAL; Jiřina BÁRTOVÁ; Hana POSKEROVÁ; Jan VOKURKA; Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Apolipoprotein E gene polymorphisms in chronic periodontitis. In \textit{FEBS CONGRESS 2013, Sankt Petersburg, Russia, FEBS Journal, vol. 280, suppl. 1, pp. 457.}. 2013. ISSN~1742-4658.

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