Intracellular distribution of the deltaNp73 protein isoform in
medulloblastoma cells: a study with newly generated rabbit
polyclonal antibodies

J 2013

Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

VESELSKÁ, Renata; Jakub NERADIL; Marta NEKULOVÁ; Lucia DOBRUCKÁ; Bořivoj VOJTĚŠEK et. al.

Základní údaje

Originální název

Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies

Autoři

VESELSKÁ, Renata; Jakub NERADIL; Marta NEKULOVÁ; Lucia DOBRUCKÁ; Bořivoj VOJTĚŠEK; Jaroslav ŠTĚRBA a Karel ZITTERBART

Vydání

Histology and Histopathology, 2013, 0213-3911

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.236

Kód RIV

RIV/00216224:14310/13:00065656

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000320710900011

Klíčová slova anglicky

medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy

Štítky

AKR, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 1. 2014 15:08, prof. RNDr. Renata Veselská, Ph.D., M.Sc.

Anotace

V originále

This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.

Návaznosti

NS10218, projekt VaV

Název: Role izoforem proteinu p73 v patogenezi meduloblastomu: imunocytochemická a microarray studie

Investor: Ministerstvo zdravotnictví ČR, Role izoforem proteinu p73 v patogenezi meduloblastomu: imunocytochemická a microarray studie

Citovat

VESELSKÁ, Renata; Jakub NERADIL; Marta NEKULOVÁ; Lucia DOBRUCKÁ; Bořivoj VOJTĚŠEK; Jaroslav ŠTĚRBA a Karel ZITTERBART. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. Histology and Histopathology. 2013, roč. 28, č. 7, s. 913-924. ISSN 0213-3911.

@article{1139223,
   author = {Veselská, Renata and Neradil, Jakub and Nekulová, Marta and Dobrucká, Lucia and Vojtěšek, Bořivoj and Štěrba, Jaroslav and Zitterbart, Karel},
   article_number = {7},
   keywords = {medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy},
   language = {eng},
   issn = {0213-3911},
   journal = {Histology and Histopathology},
   title = {Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies},
   url = {http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm},
   volume = {28},
   year = {2013}
}

TY  - JOUR
ID  - 1139223
AU  - Veselská, Renata - Neradil, Jakub - Nekulová, Marta - Dobrucká, Lucia - Vojtěšek, Bořivoj - Štěrba, Jaroslav - Zitterbart, Karel
PY  - 2013
TI  - Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies
JF  - Histology and Histopathology
VL  - 28
IS  - 7
SP  - 913-924
EP  - 913-924
SN  - 02133911
KW  - medulloblastoma;deltaNp73;immunocytochemistry;immunoblotting;transmission electron microscopy
UR  - http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
L2  - http://www.hh.um.es/Abstracts/Vol_28/28_7/28_7_913.htm
N2  - This study is focused on the intracellular localization of Delta Np73 in medulloblastoma cell lines. Due to a lack of suitable anti-Delta Np73 antibodies, we developed two new rabbit polyclonal antibodies, Delta Np7326 and Delta Np73-27, with sufficient specificity, as demonstrated by immunodetection methods using transiently transfected cell lines. Both of these new antibodies were subsequently used for analysis of the Delta Np73 distribution in medulloblastoma cells using immunofluorescence, immunoblotting and immunogold labeling for transmission electron microscopy. We found a nuclear localization of the Delta Np73 isoform in all of the medulloblastoma cell lines included in this study. Furthermore, a non-random accumulation of the Delta Np73 isoform near the cell nuclei was observable in all of these cell lines. By double-labeling with Delta Np73 and golgin-97, we showed the co-localization of the Delta Np73 isoform with the Golgi apparatus.
ER  -

VESELSKÁ, Renata; Jakub NERADIL; Marta NEKULOVÁ; Lucia DOBRUCKÁ; Bořivoj VOJTĚŠEK; Jaroslav ŠTĚRBA a Karel ZITTERBART. Intracellular distribution of the deltaNp73 protein isoform in medulloblastoma cells: a study with newly generated rabbit polyclonal antibodies. \textit{Histology and Histopathology}. 2013, roč.~28, č.~7, s.~913-924. ISSN~0213-3911.

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