The effect of cellular environment and p53 status on the mode
of action of the platinum derivative LA-12

J 2010

The effect of cellular environment and p53 status on the mode of action of the platinum derivative LA-12

ROUBALOVÁ, Eva; Veronika KVARDOVÁ; Roman HRSTKA; Šárka BOŘILOVÁ; Eva MICHALOVÁ et al.

Základní údaje

Originální název

The effect of cellular environment and p53 status on the mode of action of the platinum derivative LA-12

Autoři

ROUBALOVÁ, Eva; Veronika KVARDOVÁ; Roman HRSTKA; Šárka BOŘILOVÁ; Eva MICHALOVÁ; Lenka ZDRAŽILOVÁ DUBSKÁ; Petra SCHARTLMUELLER; Petr SOVA a Bořivoj VOJTĚŠEK

Vydání

Investigational New Drugs, DORDRECHT, SPRINGER, 2010, 0167-6997

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.007

Označené pro přenos do RIV

DOI

https://doi.org/10.1007/s10637-009-9270-4

UT WoS

000277942700008

Změněno: 15. 10. 2014 14:29, MUDr. RNDr. Michal Řiháček, Ph.D., EuSpLM

Anotace

V originále

In this study, we characterized the effects of LA-12 on tumor cell lines possessing wild type p53 and on p53-deficient/mutant cell lines and the results were compared to those obtained using cisplatin. We have determined changes of p53 levels, of its transcriptional activity, of its posttranscriptional modifications and the effect of the treatment on the cell cycle, on the induction of apoptosis and on gene expression. LA-12 induces weak accumulation of both transcriptionally active p53 tumor suppressor and of p21(WAF1/CIP1) protein. LA-12 and cisplatin also significantly differ in their effects on apoptosis and cell cycle and on gene expression spectra in studied cell lines. LA-12 induces higher apoptosis levels in comparison with those induced by cisplatin, especially in p53-deficient H1299 cells and in MCF-7DD cells with transcriptionally inactive p53. We suggest that LA-12-mediated apoptosis is not fully dependent on p53. This confirms the therapeutic potential of LA-12 as a more potent cytostatic agent for both tumor cells expressing wild type p53 and for p53-deficient or mutant cells.

Citovat

ROUBALOVÁ, Eva; Veronika KVARDOVÁ; Roman HRSTKA; Šárka BOŘILOVÁ; Eva MICHALOVÁ; Lenka ZDRAŽILOVÁ DUBSKÁ; Petra SCHARTLMUELLER; Petr SOVA a Bořivoj VOJTĚŠEK. The effect of cellular environment and p53 status on the mode of action of the platinum derivative LA-12. Investigational New Drugs. DORDRECHT: SPRINGER, 2010, roč. 28, č. 4, s. 445-453. ISSN 0167-6997. Dostupné z: https://doi.org/10.1007/s10637-009-9270-4.

@article{1202741,
   author = {Roubalová, Eva and Kvardová, Veronika and Hrstka, Roman and Bořilová, Šárka and Michalová, Eva and Zdražilová Dubská, Lenka and Schartlmueller, Petra and Sova, Petr and Vojtěšek, Bořivoj},
   article_location = {DORDRECHT},
   article_number = {4},
   doi = {https://doi.org/10.1007/s10637-009-9270-4},
   issn = {0167-6997},
   journal = {Investigational New Drugs},
   title = {The effect of cellular environment and p53 status on the mode of action of the platinum derivative LA-12},
   volume = {28},
   year = {2010}
}

TY  - JOUR
ID  - 1202741
AU  - Roubalová, Eva - Kvardová, Veronika - Hrstka, Roman - Bořilová, Šárka - Michalová, Eva - Zdražilová Dubská, Lenka - Schartlmueller, Petra - Sova, Petr - Vojtěšek, Bořivoj
PY  - 2010
TI  - The effect of cellular environment and p53 status on the mode of action of the platinum derivative LA-12
JF  - Investigational New Drugs
VL  - 28
IS  - 4
SP  - 445-453
EP  - 445-453
PB  - SPRINGER
SN  - 01676997
N2  - In this study, we characterized the effects of LA-12 on tumor cell lines possessing wild type p53 and on p53-deficient/mutant cell lines and the results were compared to those obtained using cisplatin. We have determined changes of p53 levels, of its transcriptional activity, of its posttranscriptional modifications and the effect of the treatment on the cell cycle, on the induction of apoptosis and on gene expression. LA-12 induces weak accumulation of both transcriptionally active p53 tumor suppressor and of p21(WAF1/CIP1) protein. LA-12 and cisplatin also significantly differ in their effects on apoptosis and cell cycle and on gene expression spectra in studied cell lines. LA-12 induces higher apoptosis levels in comparison with those induced by cisplatin, especially in p53-deficient H1299 cells and in MCF-7DD cells with transcriptionally inactive p53. We suggest that LA-12-mediated apoptosis is not fully dependent on p53. This confirms the therapeutic potential of LA-12 as a more potent cytostatic agent for both tumor cells expressing wild type p53 and for p53-deficient or mutant cells.
ER  -

ROUBALOVÁ, Eva; Veronika KVARDOVÁ; Roman HRSTKA; Šárka BOŘILOVÁ; Eva MICHALOVÁ; Lenka ZDRAŽILOVÁ DUBSKÁ; Petra SCHARTLMUELLER; Petr SOVA a Bořivoj VOJTĚŠEK. The effect of cellular environment and p53 status on the mode of action of the platinum derivative LA-12. \textit{Investigational New Drugs}. DORDRECHT: SPRINGER, 2010, roč.~28, č.~4, s.~445-453. ISSN~0167-6997. Dostupné z: https://doi.org/10.1007/s10637-009-9270-4.

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