Expression of nestin, CD133 and ABCG2 in relation to the
clinical outcome in pediatric sarcomas

J 2016

Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas

ZAMBO, Iva, Markéta HERMANOVÁ, Danica ZAPLETALOVÁ, Jan ŠKODA, Peter MÚDRY et. al.

Základní údaje

Originální název

Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas

Autoři

ZAMBO, Iva (203 Česká republika, domácí), Markéta HERMANOVÁ (203 Česká republika, domácí), Danica ZAPLETALOVÁ (703 Slovensko, domácí), Jan ŠKODA (203 Česká republika, domácí), Peter MÚDRY (203 Česká republika, domácí), Michal KÝR (203 Česká republika, domácí), Karel ZITTERBART (203 Česká republika, domácí), Jaroslav ŠTĚRBA (203 Česká republika, domácí) a Renata VESELSKÁ (203 Česká republika, garant, domácí)

Vydání

Cancer Biomarkers, Amsterdam, IOS Press, 2016, 1574-0153

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30209 Paediatrics

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.274

Kód RIV

RIV/00216224:14310/16:00088884

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3233/CBM-160623

UT WoS

000381114300014

Klíčová slova anglicky

rhabdomyosarcoma;Ewing sarcoma;osteosarcoma;cancer stem cell markers;prognosis

Štítky

AKR, EL OK, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2017 18:45, Ing. Andrea Mikešková

Anotace

V originále

Nestin, CD133 and ABCG2 are recently discussed as putative markers, co-expression of which might determine a cancer stem cell (CSC) phenotype in sarcomas. Our study is focused on immunohistochemical analysis of nestin, CD133 and ABCG2 expression in rhabdomyosarcoma, Ewing sarcoma and osteosarcoma. Furthermore, we also analyzed the possible correlation of nestin, CD133 and ABCG2 expression levels with the patient outcome to identify potential prognostic values of these three putative CSC markers in the same cohorts. Using immunohistochemistry, expression of nestin, CD133 and ABCG2 was analyzed in 24 rhabdomyosarcoma, 22 Ewing sarcoma and 10 osteosarcoma tissue samples and expression levels of these markers were correlated with clinical outcome. High nestin levels indicate poor prognosis in patients with Ewing sarcoma (P = 0.001), and high CD133 expression is associated with shorter survival in rhabdomyosarcoma patients (P = 0.002). In contrast, no significant relationship was found between ABCG2 expression and the clinical outcome. Our analysis represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors.

Návaznosti

NT13443, projekt VaV

Název: Identifikace a charakterizace nádorových kmenových buněk u sarkomů dětského věku.

Citovat

ZAMBO, Iva, Markéta HERMANOVÁ, Danica ZAPLETALOVÁ, Jan ŠKODA, Peter MÚDRY, Michal KÝR, Karel ZITTERBART, Jaroslav ŠTĚRBA a Renata VESELSKÁ. Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas. Cancer Biomarkers. Amsterdam: IOS Press, 2016, roč. 17, č. 1, s. 107-116. ISSN 1574-0153. Dostupné z: https://dx.doi.org/10.3233/CBM-160623.

@article{1354608,
   author = {Zambo, Iva and Hermanová, Markéta and Zapletalová, Danica and Škoda, Jan and Múdry, Peter and Kýr, Michal and Zitterbart, Karel and Štěrba, Jaroslav and Veselská, Renata},
   article_location = {Amsterdam},
   article_number = {1},
   doi = {http://dx.doi.org/10.3233/CBM-160623},
   keywords = {rhabdomyosarcoma;Ewing sarcoma;osteosarcoma;cancer stem cell markers;prognosis},
   language = {eng},
   issn = {1574-0153},
   journal = {Cancer Biomarkers},
   title = {Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas},
   volume = {17},
   year = {2016}
}

TY  - JOUR
ID  - 1354608
AU  - Zambo, Iva - Hermanová, Markéta - Zapletalová, Danica - Škoda, Jan - Múdry, Peter - Kýr, Michal - Zitterbart, Karel - Štěrba, Jaroslav - Veselská, Renata
PY  - 2016
TI  - Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas
JF  - Cancer Biomarkers
VL  - 17
IS  - 1
SP  - 107-116
EP  - 107-116
PB  - IOS Press
SN  - 15740153
KW  - rhabdomyosarcoma;Ewing sarcoma;osteosarcoma;cancer stem cell markers;prognosis
N2  - Nestin, CD133 and ABCG2 are recently discussed as putative markers, co-expression of which might determine a cancer stem cell (CSC) phenotype in sarcomas. Our study is focused on immunohistochemical analysis of nestin, CD133 and ABCG2 expression in rhabdomyosarcoma, Ewing sarcoma and osteosarcoma. Furthermore, we also analyzed the possible correlation of nestin, CD133 and ABCG2 expression levels with the patient outcome to identify potential prognostic values of these three putative CSC markers in the same cohorts. Using immunohistochemistry, expression of nestin, CD133 and ABCG2 was analyzed in 24 rhabdomyosarcoma, 22 Ewing sarcoma and 10 osteosarcoma tissue samples and expression levels of these markers were correlated with clinical outcome. High nestin levels indicate poor prognosis in patients with Ewing sarcoma (P = 0.001), and high CD133 expression is associated with shorter survival in rhabdomyosarcoma patients (P = 0.002). In contrast, no significant relationship was found between ABCG2 expression and the clinical outcome. Our analysis represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors.
ER  -

ZAMBO, Iva, Markéta HERMANOVÁ, Danica ZAPLETALOVÁ, Jan ŠKODA, Peter MÚDRY, Michal KÝR, Karel ZITTERBART, Jaroslav ŠTĚRBA a Renata VESELSKÁ. Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas. \textit{Cancer Biomarkers}. Amsterdam: IOS Press, 2016, roč.~17, č.~1, s.~107-116. ISSN~1574-0153. Dostupné z: https://dx.doi.org/10.3233/CBM-160623.

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