| TRANTÍREK, Lukáš, Milan URBÁŠEK, Richard ŠTEFL, Juli FEIGON a Vladimír SKLENÁŘ. A Method for Direct Determination of Helical Parameters in Nucleic Acids Using Residual Dipolar Couplings. Online. Journal of the American Chemical Society. Washington, D.C.: American Chemical Society, 2000, roč. 122, č. 42, s. 10454-10455. ISSN 0002-7863. [citováno 2024-04-23] |
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@article{345311,
author = {Trantírek, Lukáš and Urbášek, Milan and Štefl, Richard and Feigon, Juli and Sklenář, Vladimír},
article_location = {Washington, D.C.},
article_number = {42},
keywords = {residual dipolar coupling; helical parameter; principal order frame; DNA; RNA; NMR; structure},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {A Method for Direct Determination of Helical Parameters in Nucleic Acids Using Residual Dipolar Couplings},
volume = {122},
year = {2000}
}
TY - JOUR
ID - 345311
AU - Trantírek, Lukáš - Urbášek, Milan - Štefl, Richard - Feigon, Juli - Sklenář, Vladimír
PY - 2000
TI - A Method for Direct Determination of Helical Parameters in Nucleic Acids Using Residual Dipolar Couplings
JF - Journal of the American Chemical Society
VL - 122
IS - 42
SP - 10454
EP - 10454
PB - American Chemical Society
SN - 00027863
KW - residual dipolar coupling
KW - helical parameter
KW - principal order frame
KW - DNA
KW - RNA
KW - NMR
KW - structure
N2 - Helical parameters describing the base-stacking and base-pairing interactions in nucleic acids are not accessible directly from X-ray or NMR experimental data. Their values can be obtained only by the analysis of refined structures. This Communication presents a novel approach for direct determination of rotational helical parameters without a structure refinement. The METHADON (MEthod for deTermination of HelicAl parameters using Dipolar cOupliNgs) procedure is based on the Saupe order matrix analysis of at least five NMR residual dipolar couplings per base and takes advantage of the restricted mobility and rigid (and well-defined) geometry of purine and pyrimidine bases. The method is demonstrated on the DNA dodecamer d(CGCGAATTCGCG)
ER -
TRANTÍREK, Lukáš, Milan URBÁŠEK, Richard ŠTEFL, Juli FEIGON a Vladimír SKLENÁŘ. A Method for Direct Determination of Helical Parameters in Nucleic Acids Using Residual Dipolar Couplings. Online. \textit{Journal of the American Chemical Society}. Washington, D.C.: American Chemical Society, 2000, roč.~122, č.~42, s.~10454-10455. ISSN~0002-7863. [citováno 2024-04-23]
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