Immunomicroscopical observation on the nervous system of adult
Eudiplozoon nippnicum (Monogenea: Diplozoidae)

J 2001

Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)

ZURAWSKI, Tomáš; A. MOUSLEY; G.R. MAIR; G.P. BRENNAN; A.G. MAULE et. al.

Základní údaje

Originální název

Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)

Autoři

ZURAWSKI, Tomáš; A. MOUSLEY; G.R. MAIR; G.P. BRENNAN; A.G. MAULE; Milan GELNAR a D.W. HALTON

Vydání

International Journal for Parasitology, Australia, Elsevier, 2001, 0020-7519

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.814

Kód RIV

RIV/00216224:14310/01:00004774

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

monogenean neurobiology; cholinesterase; serotonin; neuropeptides; confocal microscopy; immunogold labeling

Štítky

Cholinesterase, confocal microscopy, immunogold labeling, monogenean neurobiology, neuropeptides, serotonin

Změněno: 28. 11. 2001 18:36, prof. RNDr. Milan Gelnar, CSc.

Anotace

V originále

Neuronal pathways have been examined in adult Eudiplozoon nipponicum (Monogenea: Diplozoidae) using cytochemistry interfaced with confocal scanning laser microscopy, in an attempt to ascertain the status of the nervous system. Peptidergic and serotinergic innervation was demonstrated by indirect immunocytochemistry and cholinnergic componets by enzyme cytochemical methodology; post-embeding electron microscopical immunogold labelling revealed neuropeptide immunoreactivity at the subcellular level. All three classes of neuronal mediators were identified throught both central and peripheral elements of a well-differentiated orthogonal nervous systems.There was considerable overlap in the staining patterns for cholinergic and peptinergic compoments; while dual immunostaining revealed serotonin immunoreactivity to be largely confined to a separate set of neurons. The subcellular distribution of immunoreactivity to the flatworm neuropeptide, GYIRFamide, confirmed neuropeptide localisation in dense-cored vesicles in the majority of the axons and terminal varicosities of both central and peripheral nervous systems. Result revealed an extensive and chemically diverse nervous system and suggest that pairing of individuals involves fusion of central nerve elements; it is likely also that there is continuity between the peripheral nervous systems of the two partner worms.

Návaznosti

GA524/00/0844, projekt VaV

Název: Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Investor: Grantová agentura ČR, Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

ZURAWSKI, Tomáš; A. MOUSLEY; G.R. MAIR; G.P. BRENNAN; A.G. MAULE; Milan GELNAR a D.W. HALTON. Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae). International Journal for Parasitology. Australia: Elsevier, 2001, roč. 31, -, s. 783-792. ISSN 0020-7519.

@article{378245,
   author = {Zurawski, Tomáš and Mousley, A. and Mair, G.R. and Brennan, G.P. and Maule, A.G. and Gelnar, Milan and Halton, D.W.},
   article_location = {Australia},
   article_number = {-},
   keywords = {monogenean neurobiology; cholinesterase; serotonin; neuropeptides; confocal microscopy; immunogold labeling},
   language = {eng},
   issn = {0020-7519},
   journal = {International Journal for Parasitology},
   title = {Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)},
   volume = {31},
   year = {2001}
}

TY  - JOUR
ID  - 378245
AU  - Zurawski, Tomáš - Mousley, A. - Mair, G.R. - Brennan, G.P. - Maule, A.G. - Gelnar, Milan - Halton, D.W.
PY  - 2001
TI  - Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)
JF  - International Journal for Parasitology
VL  - 31
IS  - -
SP  - 783
EP  - 783
PB  - Elsevier
SN  - 00207519
KW  - monogenean neurobiology
KW  - cholinesterase
KW  - serotonin
KW  - neuropeptides
KW  - confocal microscopy
KW  - immunogold labeling
N2  - Neuronal pathways have been examined in adult Eudiplozoon nipponicum (Monogenea: Diplozoidae) using cytochemistry interfaced with confocal scanning laser microscopy, in an attempt to ascertain the status of the nervous system. Peptidergic and serotinergic innervation was demonstrated by indirect immunocytochemistry and cholinnergic componets by enzyme cytochemical methodology; post-embeding electron microscopical immunogold labelling revealed neuropeptide immunoreactivity at the subcellular level. All three classes of neuronal mediators were identified throught both central and peripheral elements of a well-differentiated orthogonal nervous systems.There was considerable overlap in the staining patterns for cholinergic and peptinergic compoments; while dual immunostaining revealed serotonin immunoreactivity to be largely confined to a separate set of neurons. The subcellular distribution of immunoreactivity to the flatworm neuropeptide, GYIRFamide, confirmed neuropeptide localisation in dense-cored vesicles in the majority of the axons and terminal varicosities of both central and peripheral nervous systems. Result revealed an extensive and chemically diverse nervous system and suggest that pairing of individuals involves fusion of central nerve elements; it is likely also that there is continuity between the peripheral nervous systems of the two partner worms.
ER  -

ZURAWSKI, Tomáš; A. MOUSLEY; G.R. MAIR; G.P. BRENNAN; A.G. MAULE; Milan GELNAR a D.W. HALTON. Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae). \textit{International Journal for Parasitology}. Australia: Elsevier, 2001, roč.~31, -, s.~783-792. ISSN~0020-7519.

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