| NAGATA, Yuji, Zbyněk PROKOP, Soňa MARVANOVÁ, Jana SÝKOROVÁ, Marta MONINCOVÁ, Masataka TSUDA a Jiří DAMBORSKÝ. Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB. Applied and Environmental Microbiology. roč. 69, č. 4, s. 2349-2355. ISSN 1098-5336. 2003. |
Další formáty:
BibTeX
LaTeX
RIS
@article{485836,
author = {Nagata, Yuji and Prokop, Zbyněk and Marvanová, Soňa and Sýkorová, Jana and Monincová, Marta and Tsuda, Masataka and Damborský, Jiří},
article_number = {4},
keywords = {MUTANT; EVOLUTION; MYCOBACTERIUM; DEHALOGENATION; BIODEGRADATION},
language = {eng},
issn = {1098-5336},
journal = {Applied and Environmental Microbiology},
title = {Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB},
url = {http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/aem03a.html},
volume = {69},
year = {2003}
}
TY - JOUR
ID - 485836
AU - Nagata, Yuji - Prokop, Zbyněk - Marvanová, Soňa - Sýkorová, Jana - Monincová, Marta - Tsuda, Masataka - Damborský, Jiří
PY - 2003
TI - Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB
JF - Applied and Environmental Microbiology
VL - 69
IS - 4
SP - 2349-2355
EP - 2349-2355
SN - 10985336
KW - MUTANT
KW - EVOLUTION
KW - MYCOBACTERIUM
KW - DEHALOGENATION
KW - BIODEGRADATION
UR - http://ncbr.chemi.muni.cz/~jiri/ABSTRACTS/aem03a.html
N2 - The homology model of protein Rv2579 from Mycobacterium tuberculosis H37Rv was compared with the crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 and uncovered that six out of nineteen amino acid residues which form an active site and entrance tunnel are different between LinB and Rv2579. To characterize an effect of replacements of these six amino acid residues, mutations were introduced cumulatively into the six amino acid residues of LinB. The six-fold mutant which is supposed to have an active site of Rv2579 showed haloalkane dehalogenase activity with tested haloalkanes, confirming that Rv2579 is a member of haloalkane dehalogenase protein family.
ER -
NAGATA, Yuji, Zbyněk PROKOP, Soňa MARVANOVÁ, Jana SÝKOROVÁ, Marta MONINCOVÁ, Masataka TSUDA a Jiří DAMBORSKÝ. Re-construction of mycobacterial dehalogenase Rv2579 by cumulative mutagenesis of haloalkane dehalogenase LinB. \textit{Applied and Environmental Microbiology}. roč.~69, č.~4, s.~2349-2355. ISSN~1098-5336. 2003.
|
