Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize

MASAŘÍK, Michal; René KIZEK; K.J. KRAMER; Sabina BILLOVÁ; Marie BRÁZDOVÁ; Jan VACEK; Michele BAILEY; František JELEN and A. J. HOWARD. Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2003, vol. 75, No 11, p. 2663-2669. ISSN 0003-2700.

Basic information

Original name

Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize

Name in Czech

Aplikace avidin-biotinové technologie a adsorptivní přenosové rozpouštěcí square-wave voltametrie pro detekci DNA hybridizace a avidinu v transgenní avidinové kukuřici

Authors

MASAŘÍK, Michal; René KIZEK; K.J. KRAMER; Sabina BILLOVÁ; Marie BRÁZDOVÁ; Jan VACEK; Michele BAILEY; František JELEN and A. J. HOWARD

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2003, 0003-2700

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Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 5.250

Organization unit

Faculty of Science

UT WoS

000183397500022

Keywords in English

Streptavidin; Avidin; Adorptive Transfer Stripping Voltammetry; Square-Wave Voltammetry; Biotin Binding; Transgenic Avidin Maize; Corn; Grain; Transgenic Protein Detection; Electrochemical Detection; DNA Hybridization

Tags

Adorptive Transfer Stripping Voltammetry, Avidin, Biotin Binding, Corn, DNA hybridization, electrochemical detection, Grain, Square-Wave Voltammetry, streptavidin, Transgenic Avidin Maize, Transgenic Protein Detection

Tags

International impact, Reviewed

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Abstract

In the original language

The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.

In Czech

Práce popisuje využití avidin-biotinové technologie a square wave voltametrie (SWV) v kombinaci s adsorptivní rozpouštěcí přenosovou technikou (AdTS) pro stanovení samotného avidinu a streptavidinu s použitím uhlíkové pastové elektrody a dále využití modifikované pastové elektrody pro detekci DNA hybridizace a ke stanovení avidinu v transgenní rostlině (Zea mays), do které byl vnesen gen pro avidin.

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Links

GA203/02/0422, research and development project	Name: Nové směry v elektrochemii nukleových kyselin a jejich aplikace v chemii životního prostředí Investor: Czech Science Foundation, New trends in electrochemistry of nucleic acids and their applications in enviromental chemistry
IAA1163201, research and development project	Name: Využití adsorptivní přenosové a eliminační techniky pro elektrochemickou analýzu oligonukleotidů a nukleových kyselin Investor: Academy of Sciences of the Czech Republic, Application of adsorptive transfer and elemination techniques in oligonucleotides and nucleic acids analysis.