2004
Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus
SKLÁDAL, Petr, Carla SANTOS RICARDI, Hideko YAMANAKA and Paolo COSTABasic information
Original name
Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus
Name in Czech
Piezoelektrický biosensor pro sledování hybridizace v reálném čase a pro stanovení viru hepatitidy C
Authors
SKLÁDAL, Petr (203 Czech Republic, guarantor), Carla SANTOS RICARDI (76 Brazil), Hideko YAMANAKA (76 Brazil) and Paolo COSTA (76 Brazil)
Edition
Journal of Virological Methods, Amsterdam, Elsevier, 2004, 0166-0934
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 1.729
RIV identification code
RIV/00216224:14310/04:00010106
Organization unit
Faculty of Science
UT WoS
000220770200006
Keywords in English
Piezoelectric biosesnsor; DNA; Hepatitis C; PCR; Hybridization
Changed: 18/1/2005 10:40, prof. RNDr. Petr Skládal, CSc.
V originále
The piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (4 different sequences). Thus prepared piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use.
In Czech
Piezoelektrický krystalový resonátor byl modifikován oligonukleotidovou probou a použit pro detekci virové nukleové kyseliny v seru. Biotinylované proby byly navázany na monovrstvu avidinu kovalentně vázanou na povrch zlatých elektrod. Virová RNA byla pomocí RT-PCR techniky převedena na DNA, které pak byly detekovány PZ biosensorem. Pro srovnání byly vzorky analyzovány také soupravou Amplicor firmy Roche (fotometrické stanovení na mikrodestičce).
Links
| OC 518.30, research and development project |
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