Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry

HAVLIŠ, Jan a Andrej SHEVCHENKO. Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2004, roč. 76, č. 11, s. 3029-3036, 7 s. ISSN 0003-2700.

Základní údaje

Originální název

Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry

Název anglicky

Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry

Autoři

HAVLIŠ, Jan a Andrej SHEVCHENKO

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2004, 0003-2700

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Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.450

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000221755200015

Klíčová slova anglicky

COMPARATIVE PROTEOMICS; PEPTIDE; MIXTURES; O-18; PHOSPHORYLATION; PURIFICATION; QUANTITATION; EXPRESSION; DIGESTION; STANDARDS

Štítky

COMPARATIVE PROTEOMICS, DIGESTION, Expression, MIXTURES, O-18, peptide, phosphorylation, purification, quantitation, standards

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

Anglicky

A combination of nanoelectrospray tandem mass spectrometry and O-18-labeled peptide internal standards was applied for the absolute quantification of proteins from their in-solution and in-gel tryptic digests. Although absolute quantification from in-solution digests was accurate, we observed that in-gel digestion compromised the quantification accuracy by affecting the recovery of individual peptides and, therefore, the provided estimates might be strongly influenced by the selection of reference peptides. Under optimized experimental conditions, it was possible to provide a semiquantitative estimate of the absolute amount of gel separated proteins within better than 50% error margin.