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@article{608304,
author = {Kroupová, J. and Horák, D. and Pacherník, Jiří and Dvořák, Petr and Šlouf, M.},
article_number = {2},
keywords = {hydrogel; cationic; embryonic stem cells; 2-hydroxyethyl methacrylate; 1-vinyl-2-pyrrolidone},
language = {eng},
issn = {1549-3296},
journal = {Journal of Biomedical Materials Research},
title = {Functional polymer hydrogels for embryonic stem cell support},
volume = {76},
year = {2006}
}
TY - JOUR
ID - 608304
AU - Kroupová, J. - Horák, D. - Pacherník, Jiří - Dvořák, Petr - Šlouf, M.
PY - 2006
TI - Functional polymer hydrogels for embryonic stem cell support
JF - Journal of Biomedical Materials Research
VL - 76
IS - 2
SP - 315-325
EP - 315-325
SN - 15493296
KW - hydrogel
KW - cationic
KW - embryonic stem cells
KW - 2-hydroxyethyl methacrylate
KW - 1-vinyl-2-pyrrolidone
N2 - One of the most exciting challenges in development of transplantation therapies is to repair the damaged part of the organ or tissue by transplantation of undifferentiated embryonic stem (ES) cells or their differentiated derivatives within three-dimensional polymer scaffold. To address this issue, new cationic hydrogel slabs were synthesized by bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) with ethylene dimethacrylate (EDMA) or 1-vinyl-2-pyrrolidone (VP) with N,N'-divinylethyleneurea (DVEU) or EDMA in the presence of saccharose or NaCl as a porogen. Biocompatibility was studied with the use of feeder-independent mouse ES cell line D3. When cultured for a limited period of time, ES cells retained their undifferentiated state independently of properties of the hydrogel slabs. Notably, prolonged culture in superporous hydrogel slabs initiated ES cell differentiation.
ER -
KROUPOVÁ, J., D. HORÁK, Jiří PACHERNÍK, Petr DVOŘÁK a M. ŠLOUF. Functional polymer hydrogels for embryonic stem cell support. \textit{Journal of Biomedical Materials Research}. roč.~76, č.~2, s.~315-325. ISSN~1549-3296. 2006.
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