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@proceedings{830572,
author = {Motáčková, Veronika and Šanderová, Hana and Nováček, Jiří and Žídek, Lukáš and Krásný, Libor and Sklenář, Vladimír},
booktitle = {VII Discussions in Structural Molecular Biology Nové Hrady},
keywords = {nmr; RNA polymerase; delta subunit; assignment; structure},
title = {NMR structural study of N-terminal domain of RNA polymerase delta subunit from Bacillus subtilis},
year = {2009}
}
TY - CONF
ID - 830572
AU - Motáčková, Veronika - Šanderová, Hana - Nováček, Jiří - Žídek, Lukáš - Krásný, Libor - Sklenář, Vladimír
PY - 2009
TI - NMR structural study of N-terminal domain of RNA polymerase delta subunit from Bacillus subtilis
KW - nmr
KW - RNA polymerase
KW - delta subunit
KW - assignment
KW - structure
N2 - Contrary to the well studied RNA polymerases of gram negative bacteria, RNA polymerase of Bacillus subtilis, a gram positive bacterium, contains two additional subunits, referred to as delta and omega1. A well-structured N-terminal domain of subunit delta has been overexpressed in an E. coli expression system, labeled with stable isotopes C-13 and N-15, and investigated by nuclear magnetic resonance. A standard set of triple resonance NMR experiments was measured and almost all resonances of the protein backbone were assigned. Resonance frequencies of the side-chains were assigned using 3D TOCSY- and NOESY-type spectra. Three-bond coupling constant J(HNHA) were obtained from 3D HNHA experiments. Chemical shifts of backbone nuclei, medium range NOEs, and the three-bond coupling constants were analyzed and secondary structure was predicted. Internuclear distance restraints were extracted from NOESY spectra and used in structure calculation.
ER -
MOTÁČKOVÁ, Veronika, Hana ŠANDEROVÁ, Jiří NOVÁČEK, Lukáš ŽÍDEK, Libor KRÁSNÝ a Vladimír SKLENÁŘ. NMR structural study of N-terminal domain of RNA polymerase delta subunit from Bacillus subtilis. In \textit{VII Discussions in Structural Molecular Biology Nové Hrady}. 2009. ISSN~1211-5894.
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