Targeting the G-quadruplex-forming region near the P1 promoter
in the human BCL-2 gene with the cationic porphyrin TMPyP4 and
the complementary C-rich strand

J 2009

Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand

DEL TORO, Miguel; Pavel BUČEK; Anna AVIÑÓ; Joaquim JAUMOT; Carlos GONZALES et. al.

Základní údaje

Originální název

Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand

Název česky

Zaměření sekvence G-kvadruplexu na promotoru P1 lidského genu BCL-2 pomocí kationického porfyrinu TMPyP4 a komplementární sekvence, bohaté na cytosin

Autoři

DEL TORO, Miguel; Pavel BUČEK; Anna AVIÑÓ; Joaquim JAUMOT; Carlos GONZALES; Ramon ERITJA a Raimundo GARGALLO

Vydání

Biochimie, Paris, Elsevier Masson SAS, 2009, 0300-9084

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

ScienceDirect Article DOI: 10.1016/j.biochi.2009.04.012

Impakt faktor

Impact factor: 3.897

Kód RIV

RIV/00216224:14310/09:00035680

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000267628700011

Klíčová slova anglicky

Oligonucleotides;G_quadruplex; bcl-2;TMPyP4;Spectroscopy;Multivariate analysis

Štítky

bcl-2, G_quadruplex, Multivariate analysis, Oligonucleotides, spectroscopy, TMPyP4

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 7. 2012 17:38, Mgr. Pavel Buček, Ph.D.

Anotace

ORIG CZ

V originále

The interaction of BCL-2 G-quadruplex forming sequence with cationic porphyrin TMPyP4 and its C-rich complementary strand was studied by means of molecular absorption spectroscopy and circular dichroism, experimental dates were analysed by advanced chemometric techniques based on mulitvariate analysis. Analyses resulted in proving 1:2 stoichiometry for the complex BCL-2G:TMPyP4 and equilibrium constant 5,0*10^13 M-2, that is weaker than complex of BCL-2 complementary strands, equilibrium constant of which was determined 10^7,7 M-1.

Česky

Studie interakcí oligonukleotidu bohatého na guanin, tvořícího G-kvadruplex, s kationickým porfyrinem TMPyP4 a komplementárním vláknem DNA. Experimenty byly monitorovány pomocí molek. absorpční spektroskopie a cirkulárního dichroismu, analýza dat pokročilými chemometrickými metodani založenými na multivariátní analýze. Výsledky potvrdily navrženou stechiometrii komplexu BCL-2G:TMPyP4 1:2, s konstantou stability 5,0*10^13 M-2. Duplex obou komplementárních sekvencí BCL-2 je silnější, konstanta stability 10^7,7 M-1.

Citovat

DEL TORO, Miguel; Pavel BUČEK; Anna AVIÑÓ; Joaquim JAUMOT; Carlos GONZALES; Ramon ERITJA a Raimundo GARGALLO. Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand. Biochimie. Paris: Elsevier Masson SAS, 2009, roč. 91, č. 7, s. 894-902. ISSN 0300-9084.

@article{834828,
   author = {del Toro, Miguel and Buček, Pavel and Aviñó, Anna and Jaumot, Joaquim and Gonzales, Carlos and Eritja, Ramon and Gargallo, Raimundo},
   article_location = {Paris},
   article_number = {7},
   keywords = {Oligonucleotides;G_quadruplex; bcl-2;TMPyP4;Spectroscopy;Multivariate analysis},
   language = {eng},
   issn = {0300-9084},
   journal = {Biochimie},
   title = {Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand},
   url = {http://www.sciencedirect.com/science/article/pii/S0300908409001126},
   volume = {91},
   year = {2009}
}

TY  - JOUR
ID  - 834828
AU  - del Toro, Miguel - Buček, Pavel - Aviñó, Anna - Jaumot, Joaquim - Gonzales, Carlos - Eritja, Ramon - Gargallo, Raimundo
PY  - 2009
TI  - Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand
JF  - Biochimie
VL  - 91
IS  - 7
SP  - 894-902
EP  - 894-902
PB  - Elsevier Masson SAS
SN  - 03009084
KW  - Oligonucleotides;G_quadruplex
KW  - bcl-2;TMPyP4;Spectroscopy;Multivariate analysis
UR  - http://www.sciencedirect.com/science/article/pii/S0300908409001126
N2  - The interaction of BCL-2 G-quadruplex forming sequence with cationic porphyrin TMPyP4 and its C-rich complementary strand was studied by means of molecular absorption spectroscopy and circular dichroism, experimental dates were analysed by advanced chemometric techniques based on mulitvariate analysis. Analyses resulted in proving 1:2 stoichiometry for the complex BCL-2G:TMPyP4 and equilibrium constant 5,0*10^13 M-2, that is weaker than complex of BCL-2 complementary strands, equilibrium constant of which was determined 10^7,7 M-1.
ER  -

DEL TORO, Miguel; Pavel BUČEK; Anna AVIÑÓ; Joaquim JAUMOT; Carlos GONZALES; Ramon ERITJA a Raimundo GARGALLO. Targeting the G-quadruplex-forming region near the P1 promoter in the human BCL-2 gene with the cationic porphyrin TMPyP4 and the complementary C-rich strand. \textit{Biochimie}. Paris: Elsevier Masson SAS, 2009, roč.~91, č.~7, s.~894-902. ISSN~0300-9084.

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