Tumor promoting effects of cyanobacterial extracts are
potentiated by anthropogenic contaminants - Evidence from in
vitro study.

J 2012

Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants - Evidence from in vitro study.

NOVÁKOVÁ, Kateřina; Luděk BLÁHA a Pavel BABICA

Základní údaje

Originální název

Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants - Evidence from in vitro study.

Autoři

NOVÁKOVÁ, Kateřina; Luděk BLÁHA a Pavel BABICA

Vydání

Chemosphere, OXFORD, ENGLAND, PERGAMON-ELSEVIER SCIENCE LTD, 2012, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.137

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/12:00057560

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.chemosphere.2012.04.008

UT WoS

000307158600004

Klíčová slova anglicky

Cyanobacteria; Cylindrospermopsin; Gap junctional intercellular communication; Polychlorinated biphenyls; Polycyclic aromatic hydrocarbons; Synergistic effects

Štítky

AKR, rivok

Změněno: 11. 4. 2013 21:06, Ing. Andrea Mikešková

Anotace

V originále

In the present study we investigated combined effects of anthropogenic environmental contaminants 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) and fluoranthene, cyanotoxins microcystin-LR and cylindrospermopsin, and extracts of laboratory cultures of cyanobacteria Aphanizomenon gracile and Cylindrospermopsis raciborskii, on GJIC in the rat liver epithelial cell line WB-F344. Binary mixtures of PCB 153 with fluoranthene and the mixtures of the two cyanobacterial strains elicited simple additive effects on GJIC after 30 min exposure, whereas microcystin-LR and cylindrospermopsin neither inhibited GJIC nor altered effects of PCB 153 or fluoranthene. However, synergistic effects were observed in the cells exposed to binary mixtures of anthropogenic contaminants (PCB 153 or fluoranthene) and cyanobacterial extracts. The synergistic effects were especially pronounced after prolonged (6-24 h) co-exposure to fluoranthene and A. gracile extract, when mixture caused nearly complete GJIC inhibition, while none of the individual components caused any downregulation of GJIC at the same concentration and exposure time. The effects of cyanobacterial extracts were independent of microcystin-LR or cylindrospermopsin, which were not detected in cyanobacterial biomass. It provides further evidence on the presence of unknown tumor promoting metabolites in cyanobacteria. Clear potentiation of the GJIC inhibition observed in the mixtures of two anthropogenic contaminants and cyanobacteria highlight the importance of combined toxic effects of chemicals in complex environmental mixtures.

Návaznosti

ED0001/01/01, projekt VaV

Název: CETOCOEN

GA524/08/0496, projekt VaV

Název: Mechanismy nádorové promoce metabolitů toxických sinic

Investor: Grantová agentura ČR, Mechanismy nádorové promoce metabolitů toxických sinic

Citovat

NOVÁKOVÁ, Kateřina; Luděk BLÁHA a Pavel BABICA. Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants - Evidence from in vitro study. Chemosphere. OXFORD, ENGLAND: PERGAMON-ELSEVIER SCIENCE LTD, 2012, roč. 89, č. 1, s. 30-37. ISSN 0045-6535. Dostupné z: https://doi.org/10.1016/j.chemosphere.2012.04.008.

@article{991036,
   author = {Nováková, Kateřina and Bláha, Luděk and Babica, Pavel},
   article_location = {OXFORD, ENGLAND},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.chemosphere.2012.04.008},
   keywords = {Cyanobacteria; Cylindrospermopsin; Gap junctional intercellular communication; Polychlorinated biphenyls; Polycyclic aromatic hydrocarbons; Synergistic effects},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants - Evidence from in vitro study.},
   volume = {89},
   year = {2012}
}

TY  - JOUR
ID  - 991036
AU  - Nováková, Kateřina - Bláha, Luděk - Babica, Pavel
PY  - 2012
TI  - Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants - Evidence from in vitro study.
JF  - Chemosphere
VL  - 89
IS  - 1
SP  - 30-37
EP  - 30-37
PB  - PERGAMON-ELSEVIER SCIENCE LTD
SN  - 00456535
KW  - Cyanobacteria
KW  - Cylindrospermopsin
KW  - Gap junctional intercellular communication
KW  - Polychlorinated biphenyls
KW  - Polycyclic aromatic hydrocarbons
KW  - Synergistic effects
N2  - In the present study we investigated combined effects of anthropogenic environmental contaminants 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) and fluoranthene, cyanotoxins microcystin-LR and cylindrospermopsin, and extracts of laboratory cultures of cyanobacteria Aphanizomenon gracile and Cylindrospermopsis raciborskii, on GJIC in the rat liver epithelial cell line WB-F344. Binary mixtures of PCB 153 with fluoranthene and the mixtures of the two cyanobacterial strains elicited simple additive effects on GJIC after 30 min exposure, whereas microcystin-LR and cylindrospermopsin neither inhibited GJIC nor altered effects of PCB 153 or fluoranthene. However, synergistic effects were observed in the cells exposed to binary mixtures of anthropogenic contaminants (PCB 153 or fluoranthene) and cyanobacterial extracts. The synergistic effects were especially pronounced after prolonged (6-24 h) co-exposure to fluoranthene and A. gracile extract, when mixture caused nearly complete GJIC inhibition, while none of the individual components caused any downregulation of GJIC at the same concentration and exposure time. The effects of cyanobacterial extracts were independent of microcystin-LR or cylindrospermopsin, which were not detected in cyanobacterial biomass. It provides further evidence on the presence of unknown tumor promoting metabolites in cyanobacteria. Clear potentiation of the GJIC inhibition observed in the mixtures of two anthropogenic contaminants and cyanobacteria highlight the importance of combined toxic effects of chemicals in complex environmental mixtures.
ER  -

NOVÁKOVÁ, Kateřina; Luděk BLÁHA a Pavel BABICA. Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants - Evidence from in vitro study. \textit{Chemosphere}. OXFORD, ENGLAND: PERGAMON-ELSEVIER SCIENCE LTD, 2012, roč.~89, č.~1, s.~30-37. ISSN~0045-6535. Dostupné z: https://doi.org/10.1016/j.chemosphere.2012.04.008.

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