VSBC0221p Biochemistry I - lecture

Faculty of Medicine
Spring 2016
Extent and Intensity
2/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
RNDr. Hana Paulová, CSc. (lecturer)
Mgr. Jiří Slanina, Ph.D. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Ing. Martina Čarnecká, Ph.D. (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
MUDr. Miroslava Hlaváčová, Ph.D. (assistant)
PharmDr. Katarína Kostolanská (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Mgr. Milena Matejovičová, Ph.D. (assistant)
Lenka Nerudová (assistant)
Mgr. Kristýna Nosková, Ph.D. (assistant)
Mgr. Lucie Novotná (assistant)
Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Anna Pleskačová, Ph.D. (assistant)
Mgr. Gabriela Přibyl Dovrtělová, Ph.D. (assistant)
Mgr. Roman Sándor (assistant)
PharmDr. Kristýna Šebrlová, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
PharmDr. Miroslav Turjap, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Prerequisites (in Czech)
VSLC011s Med. Chem. - seminar && VSLC011c Medical chemistry practice && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. The graduate is acquainted with properties and function of enzymes, is oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences.Understands the principles of cell bioenegetics.Become familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport.Has knowledge on proteosynthesis including processes replication,transcription, translation and posttranslantion modifications.Understands the relation between the structure and function of proteins. Is acquinted with function of hemoglobine in oxygen transport.Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.
Syllabus
  • Membrane structure, the assembly and recycling of membranes. Specialized structures of plasma membrane – lipid rafts, caveols, tight junctions. membrane transport. Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Structure of haemoglobin, structure-function relationships (the oxygen saturation curve, inducement of haemoglobin saturation and oxygen transport. Bohr effect. Normal haemoglobin types in blood, haemoglobin concentration. Other forms (glycohaemoglobin, methaemoglobin, carboxyhaemoglobin) and abnormal haemoglobins. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.
Literature
    required literature
  • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Barevný atlas biochemie. Grada. Praha, 2012. 498 pp. ISBN 978-80-247-2977-0. info
  • KOOLMAN, Jan and Klaus-Heinrich ROEHM. Color Atlas of Biochemistry. 3rd ed. Georg Thieme Verlag, 2013. ISBN 978-3-13-100373-7. info
    recommended literature
  • LEDVINA, Miroslav, Alena STOKLASOVÁ and Jaroslav CERMAN. Biochemie pro studující medicíny, I. a II. díl. Druhé vydání. Praha: Karolinum, 2009. 546 pp. ISBN 978-80-246-1414-4. info
Teaching methods
Teaching form are lectures. Supplementary subjects are seminars VSBC021s
Assessment methods
Student must have course-unit credit of seminar in the day of exam. Examination has writen and oral part. The examination has two parts. The computer test (ROPOT) is the first part of the examination The test consists of 25 questions. An essential knowledge ranging over all studied topics is required. Above all, the structural formulas of compounds with biochemical significance, chemical equations of elemental reactions in major metabolic pathways (with particular formulas), and basic principles of metabolic control. Only those students who gain 14 correct answers at a minimum will be permitted to sit for the oral examination. The oral part of examination: Students select three questions and have about 30 minutes for the written preparation. They should summarize their answers as concisely and accurately as possible. It is recommended to follow these items: - to write a brief synopsis emphasizing the main ideas. - to draw metabolic pathways in structural formulas with a short comment. - where appropriate, to draw a picture (e.g. membranes, respiratory chain etc.). A good and concise preparation reflects the students’ knowledge and understanding the biochemistry and will be considered in the final classification.
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on completion of the course: zkoušku lze konat až po absolvování zkoušky z Lékařské chemie
The course is taught annually.
The course is taught: every week.
Information on course enrolment limitations: Přihlášení ke zkoušce je podmíněno vykonáním zkoušky z Lékařské chemie.
Listed among pre-requisites of other courses
Teacher's information
http://www.med.muni.cz/toCP1250.cs/biochem/vyuka.html
The course is also listed under the following terms Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Autumn 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015.
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