aVLKB091 Clinical Biochemistry

Faculty of Medicine
autumn 2023
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: k (colloquium).
Taught in person.
MUDr. Lenka Gescheidtová, Ph.D. (lecturer)
MUDr. Michaela Králíková, Ph.D. (lecturer)
MUDr. Ondřej Kyselák, Ph.D., EuSpLM (lecturer)
MUDr. Jan Novák, Ph.D. (lecturer)
MUDr. Lukáš Opatřil, Ph.D. (lecturer)
MUDr. RNDr. Michal Řiháček, Ph.D. (lecturer)
prof. MUDr. Vladimír Soška, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
prof. MUDr. Dalibor Valík, Ph.D., DABCC (lecturer)
Mgr. Ondřej Wiewiorka, Ph.D. (lecturer)
MUDr. Ing. David Zeman, Ph.D. (lecturer)
Marcela Nečasová (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Contact Person: MUDr. Michaela Králíková, Ph.D.
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Tue 14:30–17:20 A21/107
Prerequisites (in Czech)
aVLPF0622p Pathophysiology II - lecture || aZLPF0622p Pathophysiology II - lecture
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After this course the participant should be able to: - choose biochemical tests suitable for diagnostics of diseases - know the basic problems of common biochemical diagnostic tests - know how to collect biological material and how to treat it to prevent false results - to assess reliability of laboratory results.
Learning outcomes
Student will be able to:
- purposefully indicate laboratory biochemical tests
- correctly interpret laboratory results
- evaluate laboratory tests with regard to their sensitivity and specificity
- use knowledge of the uncertainty of the evaluated parameter
- to focus on possible pre-analytical influences and interferences
  • Analytical sources of variation, accuracy and precision. Diagnostic accuracy, sensitivity, specificity, and efficiency of diagnostic tests. Reference ranges, decision values. Collection and processing of specimens, special requirements, general safety rules.
  • Assessment and monitoring of nutritional status. Blood plasma proteins. Acute-phase reactants. Principles of nutritional support, enteral and parenteral nutrition. Dietary constituents providing a sufficient energy intake.
  • Energy output, nitrogen balance. Indications for nutritional support. Oxygen, transport and metabolism, tissue hypoxia.
  • Disorders of lipid and lipoprotein metabolism.
  • Ischemic heart disease, congestive cardiac failure. Potassium and magnesium homeostasis.
  • Iron and essential microelements metabolism.
  • Disturbances of water and sodium balance. Plasma and urine osmolality. Plasma electrolytes in acid-base disorders.
  • Assessment of acid-base balance. Investigating an acid-base status, indications for blood acid-base and oxygen measurements, interpretation of results.
  • Laboratory evaluation of kidney function. Biochemical monitoring of acute and chronic renal failure, and of patients receiving haemodialysis. Urine sediment.
  • Investigation of liver and biliary tract diseases. Signs of the liver injury, differentiating of hyperbilirubinaemias, syndrome of biliary obstruction.
  • Clinical biochemistry of the GIT. Gastric acid secretion, maldigestion and malabsorption, assessment of pancreatic function.
  • Disorders of saccharide metabolism, diabetes mellitus.
  • Calcium and phosphate metabolism. Biochemical markers of bone metabolism (in the assessment of osteoporosis).
  • Uric acid, hyperuricaemia. Renal tract stones.
  • Laboratory assessment of thyroid and adrenal functions.
  • Tumour markers. Biological causes of variation of test results, effects of drugs on chemical tests.
    required literature
  • Study texts available in Information system.
  • Rajdl. D. a kol. Clinical biochemistry (e-book, formats: .pdf, .epub, .mobi) 1st ed. Prague, Karolinum Press, 2016, 426 s. ISBN: 978-80-246-3497-5. Download: https://karolinum.cz/en/books/rajdl-clinical-biochemistry-17096
    recommended literature
  • MARSHALL, William J. and S. K. BANGERT. Clinical chemistry. 6th ed. Edinburgh: Mosby, 2008. 416 s. ISBN 9780723434603. info
  • WALKER, Simon A. Lecture notes clinical biochemistry. 9th ed. Chichester, West Sussex: Wiley-Blackwell, 2013. xii, 290. ISBN 9781118272138. info
Teaching methods
Lectures, discussion of clinical cases.
Assessment methods
The colloquium is in the form of a written test.
The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
Language of instruction
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/med/autumn2023/aVLKB091