MNAB0711s Applied biochemistry

Faculty of Medicine
Autumn 2017
Extent and Intensity
0/2. 2 credit(s). Type of Completion: k (colloquium).
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
prof. MUDr. Vladimír Soška, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
Mgr. Miroslav Fiala (assistant)
MVDr. Halina Matějová (assistant)
Guaranteed by
prof. MUDr. Vladimír Soška, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Mon 2. 10. to Sun 10. 12. Mon 14:00–16:30 B11/114
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After this course the participant should be able to: - choose biochemical tests suitable for diagnostics of diseases - know the basic problems of common biochemical diagnostic tests - know how to collect biological material and how to treat it to prevent false results - to assess reliability of laboratory results.
  • Analytical sources of variation, accuracy and precision. Diagnostic accuracy, sensitivity, specificity, and efficiency of diagnostic tests. Reference ranges, decision values. Collection and processing of specimens, special requirements, general safety rules.
  • Assessment and monitoring of nutritional status. Blood plasma proteins. Acute-phase reactants. Principles of nutritional support, enteral and parenteral nutrition. Dietary constituents providing a sufficient energy intake.
  • Energy output, nitrogen balance. Indications for nutritional support. Oxygen, transport and metabolism, tissue hypoxia.
  • Disorders of lipid and lipoprotein metabolism.
  • Ischemic heart disease, congestive cardiac failure. Potassium and magnesium homeostasis.
  • Iron and essential microelements metabolism.
  • Disturbances of water and sodium balance. Plasma and urine osmolality. Plasma electrolytes in acid-base disorders.
  • Assessment of acid-base balance. Investigating an acid-base status, indications for blood acid-base and oxygen measurements, interpretation of results.
  • Laboratory evaluation of kidney function. Biochemical monitoring of acute and chronic renal failure, and of patients receiving haemodialysis. Urine sediment.
  • Investigation of liver and biliary tract diseases. Signs of the liver injury, differentiating of hyperbilirubinaemias, syndrome of biliary obstruction.
  • Clinical biochemistry of the GIT. Gastric acid secretion, maldigestion and malabsorption, assessment of pancreatic function.
  • Disorders of saccharide metabolism, diabetes mellitus.
  • Calcium and phosphate metabolism. Biochemical markers of bone metabolism (in the assessment of osteoporosis).
  • Uric acid, hyperuricaemia. Renal tract stones.
  • Laboratory assessment of thyroid and adrenal functions.
  • Tumour markers. Biological causes of variation of test results, effects of drugs on chemical tests.
    required literature
  • RACEK, Jaroslav. Klinická biochemie. 2., přeprac. vyd. Praha: Galén, 2006. 329 s. ISBN 8072623249. info
    recommended literature
  • DASTYCH, Milan. Klinická biochemie - bakalářský obor Zdravotní laborant (Clinical Biochemistry). 1. vydání. Brno: Masarykova univerzita, 2008. 232 pp. ISBN 978-80-210-4572-9. info
  • MASOPUST, Jaroslav. Klinická biochemie : požadování a hodnocení biochemických vyšetření. Část I. Vyd. 1. Praha: Karolinum, 1998. 429 s. ISBN 80-7184-648-11. info
    not specified
  • Clinical chemistry : techniques, principles, correlations. Edited by Michael L. Bishop - Edward P. Fody - Larry E. Schoeff. 6th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins, 2010. xx, 732. ISBN 9780781790451. info
  • JABOR, A. Vnitřní prostředí. 1st ed. Praha: Grada Publishing,a.s., 2008, 2009. 560 pp. ISBN 978-80-247-1221-5. info
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood - David E. Bruns - Barbara G. Sawye. 6th ed. St. Louis, Mo.: Saunders/Elsevier, 2008. xx, 952. ISBN 9780721638652. info
Teaching methods
Assessment methods
oral exam
Language of instruction
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2018, autumn 2019, autumn 2020, autumn 2021.
  • Enrolment Statistics (Autumn 2017, recent)
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