aVLMB031 Modern Imaging and Analytical Methods in Cellular Biology and Immunology

Faculty of Medicine
autumn 2018
Extent and Intensity
0/0.7/.3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jan Frič, Ph.D. (seminar tutor)
Mgr. Petra Lázničková (seminar tutor)
Oriana Lo Re, Ph.D. (seminar tutor)
Fabiana Martino, Ph.D. (seminar tutor)
Shyam Sushama Jose, Ph.D. (seminar tutor)
Mgr. Federico Tidu, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Jan Frič, Ph.D.
Department of Biology - Theoretical Departments - Faculty of Medicine
Contact Person: Mgr. Jan Frič, Ph.D.
Supplier department: Department of Biology - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
aVLMB031/A: No timetable has been entered into IS.
aVLMB031/B: No timetable has been entered into IS.
Prerequisites
VSBI0222p Biology II-lect. || aVLBI0222p Biology II - lect. || ZLBI0222p Biology II -lect. || aZLBI0222p Biology II - lecture
Students need to have cell biology knowledge at the level of 1st year of university, proven with an exam (aVLBI0222p, aZLBI0222p or Bi1700).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 20 student(s).
Current registration and enrolment status: enrolled: 16/20, only registered: 1/20
fields of study / plans the course is directly associated with
Course objectives
After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); electron microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.
Learning outcomes
After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); electron microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.
Syllabus
  • Cell separation methods (magnetic separation, FACS sorting)
  • Cell phenotype analysis using flow cytometry – basics
  • Advanced flow cytometry methods, Spectral cytometry, Mass cytometry
  • Advanced microscopy techniques - confocal and multiphoton microscopy
  • Introduction to scanning electron microscopy SEM
Literature
  • Relevant textbooks chapters and links to internet sources that will help to understand the basic principles of the methods will be provided during the course.
Teaching methods
a seminar, lab demonstration
Assessment methods
Course completion requires full atendance in the seminars and passin a final written test (5 questions on understanding the principle and use of illustrated methods - student answer in their own words, credit is awarded in case of answering at least 4 questions out of 5).
Attendance at all seminars is mandatory
Language of instruction
English
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 15.
Teacher's information
https://is.muni.cz/auth/el/1411/podzim2016/VLMB011/index.qwarp
The course is also listed under the following terms Autumn 2017, autumn 2020.
  • Enrolment Statistics (autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2018/aVLMB031