ZLHE0221c Histology and Embryology I - practice

Faculty of Medicine
Spring 2009
Extent and Intensity
0/2. 3 credit(s). Type of Completion: z (credit).
prof. MUDr. RNDr. Svatopluk Čech, DrSc. (seminar tutor)
MUDr. Jana Dumková, Ph.D. (seminar tutor)
MUDr. Lenka Krejčířová, Ph.D. (seminar tutor)
MUDr. Irena Lauschová, Ph.D. (seminar tutor)
MUDr. Ondřej Ráček (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
doc. MUDr. Jitka Šťastná, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Drahomír Horký, DrSc.
Department of Histology and Embryology - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
ZLHE0221c/21: Tue 13:40–15:20 F01B1/126
ZLHE0221c/22: Fri 14:30–16:10 F01B1/126
ZLHE0221c/23: Tue 13:40–15:20 F01B1/126
ZLHE0221c/24: Fri 14:30–16:10 F01B1/126
ZLHE0221c/40: No timetable has been entered into IS.
Prerequisites (in Czech)
ZLAN0131s Anatomy I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Main objectives of histology course are as follows: to facilitate medical students verifying of theoretical knowledge obtained during lectures on histological sections and electron micrographs of human tissues and organs; to introduce and explain principles of microscopic identification of tissues and organs; to learn students in correct using of a light microscope and interpretation of observed microscopic entities and findings. At the end of practices, students should be able to understand principles of tissue processing for the light and electron microscopy, to describe structure and ultrastructure of human cells an to know key microscopic characteristics of all important tissues of the human body. The course I is finished with study of organs of 3 body systems: circulatory, lymphatic and respratory.
  • 1. Introduction, organization of practicals, teaching aids. Introduction into histological technique. Overview of tissue processing for light and electron microscopy - explanation and film. Study of hard tissues (bones and teeth). (Explanation and film)
  • 2. Cytology: Ultrastructure of the cell nucleus (during interphase and mitosis). Ultrastructure of cell organelles (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes,peroxisomes, and the centriol). Cell inclusions. Cell surfaces and intercellular junctions. Aids: Atlas of electron micrographs.
  • 3. Blood: Blood corpuscles: Cytomorphology of erythrocytes and leukocytes. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Thrombocytes. Slide: A smear of peripheral blood stained by Pappenheim's method. Repetition I: Tissue processing for light and electron microscopy. Cytology (a test). Development of blood cells (hematopoiesis): Erythropoiesis, granulopoiesis and development of thrombocytes. Microscopic structure of the bone marrow. Slide: A bone marrow smear stained by Pappenheim's method.
  • 4. General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin–eosine-saffron /HES/ (esophagus), AZAN (prostate), impregnation (cerebellum).
  • 5. Epithelial tissue - epithelial membranes (covering epithelia). Slides: Ren, Vesica fellea, Trachea, Esophagus, Ureter, Palpebra, Skin from the finger tip. Glandular epithelium. Absorptive, respiratory and sensory epithelia. Slides: Intestinum tenue, Intestinum crassum, Glandula parotis, Glandula submandibularis, Papilla vallata (taste buds)
  • 6. Connective tissue proper ans special connetive tissues - classification and examples. Slides: Funiculus umbilicalis, Esophagus, Posterior segment of the eye, Lien, Aorta.Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, Auricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification. Repetition II:Blood and development of blood cells, connective and supporting tissues (a test).
  • 7. Muscle tissue: the smooth, akeletal and crdiac. Slides: Intestinum tenue, Intestinum crassum, Glandula parotis, Glandula submandibularis, Apex linguae, Cardiac muscle tissue (H.E. + staining according to Heidenhain)
  • 8. Nerve tissue: neuron, dendrites, neurit and synapses; neuroglia. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
  • 9. Microscopic anatomy and embryology
  • Microscopic structure of the heart and blood vessels. Capillaries. Slides: Artery of the muscular type with a vein, Aorta, Vena cava, Myocardium. Atlas of electron micrographs: Types of capillaries. Repetition III: Epithelial tissue, muscle tissue and nerve tissue (a test).
  • 10. Microscopic structure of the lymphatic system. Slides: Lymphonodus, Lien (spleen), Thymus of a young individual, Thymus of the adult (with involution changes), Palatine tonsil, Lingual tonsil.
  • 11. Microscopic structure of the respiratory system. Slides: Nasal conchae, Epiglottis, Larynx, Trachea, Pulmo.
  • ČECH, Svatopluk, Drahomír HORKÝ, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a metody vyšetřování tkání a orgánů (Histological practicals and methods of investigation of tissues and organs). První. Brno: Vydavatelství MU Brno-Kraví hora, 1998. 162 pp. ISBN 80-210-1774-0. info
Assessment methods
In the course students observe histological slides and electron micrographs in microscopic hall. A precondition for obtaining the course-unit credit is full attendance. Students are obliged to show basic knowledge of studied topic, which is checked by short oral exam or by test 3 times in the semester. Answers are classified with 100, 60, 25 or 0 points. A minimum of points for credit obtaining is 180.
Language of instruction
Further Comments
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
The course is also listed under the following terms Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022.
  • Enrolment Statistics (Spring 2009, recent)
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