PřF:Bi9250c Special Immunol. Meth. - pr. - Course Information

Bi9250c Special Immunological Methods - practical course

Extent and Intensity

0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

prof. Mgr. Lukáš Kubala, Ph.D. (lecturer), RNDr. Lenka Šindlerová, Ph.D. (deputy)

Guaranteed by

prof. Mgr. Lukáš Kubala, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. Mgr. Lukáš Kubala, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science

Prerequisites

Bi5220 Immunology && Bi5220c Immunology - pr.
Subject is connected to the basic practice course in immunology that is why students are supposed to have finished the basic practice course in immunology. The course is to extended the theory and praxis of basic immunology subject in its methods.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 14 student(s).
Current registration and enrolment status: enrolled: 13/14, only registered: 0/14, only registered with preference (fields directly associated with the programme): 0/14

fields of study / plans the course is directly associated with

there are 6 fields of study the course is directly associated with, display

Course objectives

The aim of the course is to deepen students’ theoretical knowledge in the field of immunology and to connect it with practical experience in laboratory research. Students will become familiar with modern immunological methods using the example of evaluating the immunogenicity of proteins and peptides. They will acquire the skills necessary for independently conducting experiments focused on the interactions of the immune system with various substances. The teaching focuses on developing students’ practical abilities when working with primary immune cells isolated from blood, cell cultures, multicolor flow cytometry, ELISA assays, and co-culture systems. Emphasis is placed on students’ independent work in the laboratory, the ability to interpret experimental data, and on linking theory with practice.

Learning outcomes

At the end of this course, the student will be able to understand, explain, and implement selected advanced methodologies in immunology.

Syllabus

1: Introduction to immunogenicity and preparation for laboratory work • Theoretical introduction to immunological methods used to assess the immunogenicity of substances. • Laboratory safety, preparation for experiments, familiarization with laboratory equipment. • Monocyte activation test (ATM) – basic principles, preparation of protocols • Isolation of mononuclear cells from human blood • Performing the first phase of the ATM test and preparing the ELISA method. 2: Evaluation of the activation of part of innate immunity • Performing the second part of the ATM test and evaluating the pyrogenicity of model substances. • Analysis of the production of pro-inflammatory cytokines using ELISA. • Evaluation of the experiment and discussion of the results. 3: Evaluation of the activation of adaptive immunity – T cell functional assays • Theoretical block on adaptive immunity and T-cell responses. • Isolation and preparation of T lymphocytes for functional tests. • Start of the experiment – T cell functional assays and monitoring of cell proliferation using flow cytometry and preparation of ELISA for detection of selected cytokines. 4: Co-cultivation of antigen presenting cells with T cells • End of the experiment – T cell functional assays and monitoring of cell proliferation using flow cytometry and ELISA determination of selected cytokines. • Analysis of the data obtained from the experiments. • Theoretical introduction to the function of antigen presenting cells and their role in T cell activation. • Isolation of antigen presenting cells and their preparation for co-cultivation with T cells. • Start of co-cultivation for monitoring of cell reactions using flow cytometry. 5: Interpretation of results and final presentation • End of co-cultivation and analysis of cell reactions using flow cytometry. • Analysis of the data obtained from the experiments. • Processing and presentation of the results in the form of a final report. • Discussion of the experimental results and their biological implications. • Reflection on experiments and feedback.

Literature

required literature
• Učební materiály předmětu - přednášky poskytnuté přenášejícími.

recommended literature
• MURPHY, KENNETH M.; WEAVER, CASEY and BERG, LESLIE J. Janeway's Immunobiology. 10th. New York, London: W. W. Norton & Company, 2022, 960 pp. ISBN 978-0-393-88487-6. info

Teaching methods

Short theoretical lectures, practical implementation of immunological methods, reflection on experiments and feedback.

Assessment methods

Participation in the exercises is mandatory. The exercises will be concluded in the form of a final test and submitted protocols from individual methodologies. Self-tests and reflective discussions will be conducted during the course.

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!

Listed among pre-requisites of other courses

• Bi9250cen Special Methods of Immunology – Practical Course
  (!Bi9250c) && !NOWANY(Bi9250c) && NOW(Bi5220en)  

Teacher's information

This subject will take place in the form of a block of teaching, during the examination period. The necessity of this form is given by the complexities of some time-consuming methodologies.

• Enrolment Statistics (recent)
  
• Permalink: https://is.muni.cz/course/sci/autumn2025/Bi9250c