PřF:C4840 Methods for labelling and immo - Course Information
C4840 Methods for labelling and immobilization of biomoleculesFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Taught in person.
- prof. RNDr. Petr Skládal, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
- Mon 11:00–12:50 B11/335
- basic knowledge of biochemistry and chemistry
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- Students will gain theoretical knewledge about modification of biomolecules based on conjugation reactions, introduction of labels and immobilization on surface of polymers and sensing surfaces. They will obtain overview of reagents for conjugation and labelling (enzymes, fluorophores, biotinylation, liposomes, nanoparticles).
- Learning outcomes
- Students will learn methods for conjugation of biomolecules with labels as enzymes, fluorophores and nanoparticles. They will manage principles of immobilization techniques for preparation of affinity supports and surfaces for purification and bioanalytical applications.
- 1. Modification of proteins and peptides, side chains of amino acids, prosthetic groups.
- 2. Modification of nucleotides, DNA, RNA. Modification of (poly)saccharides, glycoconjugates.
- 3. Insertion of additional reactive groups into biomolecules. Basic chemical reactions for conjugation.
- 4. Bioconjugations, homo/heterobifunctional reagents. Cleavable links.
- 5. Fluorescent labels, fluoresceins, rhodamins, coumarins, FRET.
- 6. Biotinylation, chelates, oligohistidine tags, boronate complexes. Radiolabels, nanoparticles, liposomes.
- 7. Enzyme labels.
- 8. Immobilization of biomolecules for affinity chromatography.
- 9. Activation of inert sensing surfaces - gold, platinum, graphite.
- 10. Characterization of bioconjugates.
- 11. Hapten-carrier conjugations.
- 12. Immunosensors, sensing microarrays and DNA biochips.
- recommended literature
- HERMANSON, Greg T. Bioconjugate techniques. San Diego: Academic Press, 1995. xxv, 785 s. ISBN 0-12-342336-8. info
- Teaching methods
- lecture course
- Assessment methods
- oral examination, random selection of 3 questions - solution of model problems, preparation of answers, discussion with the teacher. Min. 50% of correct answers.
- Language of instruction
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2023/C4840