Bi5596 Advanced Methods in Ecotoxicology

Faculty of Science
autumn 2017
Extent and Intensity
3/2/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
doc. RNDr. Pavel Babica, Ph.D. (lecturer)
Mgr. Lucie Bláhová, Ph.D. (lecturer)
Mgr. Karel Brabec, Ph.D. (lecturer)
doc. Mgr. Klára Hilscherová, Ph.D. (lecturer)
prof. RNDr. Jakub Hofman, Ph.D. (lecturer)
Mgr. Jiří Novák, Ph.D. (lecturer)
Mgr. Soňa Smetanová, Ph.D. (lecturer)
RNDr. Iva Sovadinová, Ph.D. (lecturer)
Mgr. Bc. Lucie Bielská, Ph.D. (seminar tutor)
RNDr. Mgr. Michal Bittner, Ph.D. (seminar tutor)
doc. RNDr. Pavel Čupr, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Luděk Bláha, Ph.D.
RECETOX - Faculty of Science
Contact Person: doc. RNDr. Pavel Babica, Ph.D.
Supplier department: RECETOX - Faculty of Science
Mon 18. 9. to Fri 15. 12. Wed 11:00–12:50 D29/252-RCX1
The course is intended primarily for Master students of Ecotoxicology specialization (preferentially in the 1st year of Master studies). An advantage is the previous attendance of at least basic ecotoxicology courses (General ecotoxicology, Ecotoxicological bioassays).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
The aim of the course is the basic principles and applications of modern techniques and approaches, which are currently being applied in experimental and theoretical ecotoxicology. In addition to basic information of the underlying principles of the methods introduced in the course lectures, students will learn how to interpret the obtained results and outcomes, and they will also get practical experience with selected methods and models in laboratory and computer excercises.
Students will acquire theoretical knowledge and practical experience with the methods for exposure assessment and modeling, molecular and cellular toxicology including omic-based techniques, biomarkers, transgenic models, high-tier assessment, effect modeling, toxicokinetic and toxicodynamic modeling.
  • Part A - Methods for exposure evaluation and assessment
  • * Bioavailability and its assessment
  • * Passive sampling
  • * Passive dosing
  • * Evaluation and modeling of chemical uptake
  • * Exposure modeling
  • Part B - Methods for effect evaluation
  • * Macromolecules and their analysis(DNA, RNA, Proteins)
  • * Metabolites - products of physiological and pathological metabolism, biomarkers
  • * Cells (bacteria, yeast, eukaryotic models), basic in vitro cultures, evaluation of effects at cellular level
  • * Eukaryotic cells in detail (structure, function)
  • * Tissues and organisms
  • * Populations, communities, ecosystems
  • Part C - Modeling
  • * Toxicokinetics
  • * Toxicodynamics
  • * TK-TD models
  • * Dynamic Energy Bugdet (DEBTOX) models
  • * SSD
    recommended literature
  • Metody molekulární biologie a bioinformatiky. Edited by Jana Zvárová - Ivan Mazura. 1. vyd. Praha: Karolinum, 2012. 343 s. ISBN 9788024621500. info
  • ANDĚL, Petr. Ekotoxikologie, bioindikace a biomonitoring. Vyd. 1. Liberec: Evernia, 2011. 243, [22]. ISBN 9788090378797. info
  • NEWMAN, Michael C. Fundamentals of ecotoxicology. 3rd ed. Boca Raton, Fla.: CRC Press, 2010. xxii, 541. ISBN 9781420067040. info
  • ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 2. dotisk 1. vydání. Brno: Masarykova univerzita, 2010. 194 pp. ISBN 978-80-210-3841-7. info
  • DEAN, John R. Bioavailability, bioaccessibility and mobility of environmental contaminants. Chichester: John Wiley & Sons, 2007. xxi, 292. ISBN 9780470025772. info
  • Basic methods in microscopy : protocols and concepts from cells : a laboratory manual. Edited by David L. Spector - Robert D. Goldman. 1st ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 2006. ix, 382. ISBN 0879697512. info
  • ALBERTS, Bruce. Molecular biology of the cell [Alberts, 2002]. 4th ed. New York: Garland Publishing, 2002. xxxiv, 146. ISBN 0-8153-3218-2. info
  • KAISER, Jamil. Bioindicators and biomarkers of environmental pollution and risk assessment. Enfield, N.H.: Science Publishers, 2001. xviii, 204. ISBN 1578081629. info
  • BEGON, Michael, John L. HARPER and Colin R. TOWNSEND. Ecology : individuals, populations and communities [Begon, 1996]. 3rd ed. Cambridge: Blackwell Science, 1996. xii, 1068. ISBN 0-632-03801-2. info
  • Fundamentals of aquatic toxicology : effects, environmental fate, and risk assessment. Edited by Gary M. Rand. 2nd ed. Boca Raton: CRC Press, 1995. xxi, 1125. ISBN 1560320915. info
  • HOFFMAN, David J. Handbook of ecotoxicology. Boca Raton: Lewis Publishers, 1995. x, 755. ISBN 0873715853. info
  • CALOW, P. Handbook of Ecotoxicology Vol. I and II. London, U.K.: Blackwell Scientific publications, 1993. info
Teaching methods
Lectures (2 h) combined with practical laboratory (experimental ecotoxicology) or computer (modeling) excercises
Assessment methods
Students will be evaluated continuously during the semester and in the final exam.

- Students achievements during the semester (lecture attendance, minitest, quality of laboratory protocols) can comprise up to 25% (up to 10 points) from the final evaluation score (max. 40 points).
* Written minitests (evaluation of theoretical knowledge prior the follow-up course lectures)
* Written tests prior the lab excercises (evaluation of theoretical knowledge required for the lab excercise)
* Evaluation of the quality of lab protocols

- Final examination - written test of 30 questions (up to 30 points).
Language of instruction
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2014, Autumn 2015, Autumn 2016.
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