F9402 Bioelectrochemistry 1

Faculty of Science
Autumn 2018
Extent and Intensity
2/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
Teacher(s)
RNDr. František Jelen, CSc. (lecturer)
Guaranteed by
prof. RNDr. Josef Humlíček, CSc.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Contact Person: RNDr. František Jelen, CSc.
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science
Timetable
Mon 17. 9. to Fri 14. 12. Wed 8:00–9:50 Kontaktujte učitele
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objective of the course is to provide the students with ability to
- apply electrochemistry methods on biomacromolecules (nucleic acids, proteins)
- list and describe reduction properties of biomacromolecules at electrodes
- list and describe adsorption properties of biomacromolecules.
Learning outcomes
Student will be able to
- apply electrochemical methods for the research of macromolecules
- to focus on issues related to electrochemistry of nucleic acids and proteins
- to suggest suitable electrochemical methods for the analysis of biomacromolecules
Syllabus
  • Principles of bioelectrochemistry
  • Electrochemical methods
  • Application of bioelectrochemistry to the determination of nucleic acids and proteins
  • New trends in nucleic acids electrochemistry
Literature
  • HASOŇ, Stanislav, Hana PIVOŇKOVÁ, Vladimír VETTERL and Miroslav FOJTA. Label-free sequence-specific DNA sensing using copper-enhanced anodic stripping of purine bases at boron-doped diamond electrodes. Analytical Chemistry. USA: AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS, 2008, 80/2008, No 7, p. 2391-2399. ISSN 0003-2700. info
  • HASOŇ, Stanislav, Vladimír VETTERL and Miroslav FOJTA. Two-dimensional condensation of pyrimidine oligonucleotides during their self-assemblies at mercury based surfaces. Electrochimica Acta. Amsterdam: Elsevier Science, 2008, vol. 53, No 1, p. 2818 - 2824. ISSN 0013-4686. info
  • AVRANAS, Antonis, Sofia KOURTIDU and Vladimír VETTERL. Adsorption of oligonucleotides A10, A25, A50 and A80 at the mercury/electrolyte interface. Colloids and Surfaces A: Physicochemical and Engineering Aspects. Amsterdam: Elsevier, 2007, vol. 295, March, p. 178-184. ISSN 0927-7765. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Amplified oligonucleotide sensing in microliter volumes containing copper ions by solution streaming. Analytical Chemistry. USA: AMER CHEMICAL SOC, 1155 16TH ST, NW, WAS, 2006, 78/2006, No 14, p. 5179-5183. ISSN 0003-2700. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Detection of synthetic oligonucleotides by alternating current voltammetry at solid amalgam surfaces. Electrochimica Acta. UK: Pergamon, 2006, vol. 51, No 24, p. 5199-5205. ISSN 0013-4686. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Microanalysis of oligodeoxynucleotides by cathodic stripping voltammetry at amalgam-alloy surfaces in the presence of copper ions. Talanta. Amsterdam: Elsevier Science, 2006, vol. 69, No 3, p. 572-580. ISSN 0039-9140. info
  • HASOŇ, Stanislav, František JELEN, Lukáš FOJT and Vladimír VETTERL. Determination of picogram quantities of oligodeoxynucleotides by stripping voltammetry at mercury modified graphite electrode surfaces. J.Electroanal.Chem. The Netherlands: Elsevier, The Netherlands, 2005, vol. 577, No 3, p. 263-272. ISSN 0022-0728. info
  • VETTERL, Vladimír and Stanislav HASOŇ. Electrochemical properties of nucleic acid components. In Electrochemistry of nucleic acids and proteins. Towards electrochemical sensors for genomics and proteomics. 1st ed. Amsterdam: Elsevier, 2005, p. 18 - 69. Perspectives in Bioanalysis, Vol.1. ISBN 0-444-52150-X. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Application of thin film mercury electrodes and solid amalgam electrodes in electrochemical analysis of the nucleic acids components: detection of the two-dimensional phase transients of adenosine. Bioelectrochemistry. Elsevier, 2004, vol. 2004, No 63, p. 37-41. ISSN 1567-5394. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Detection of phase transients in two-dimensional adlayers of adenosine at the solid amalgam electrode surfaces. Journal of Electroanalytical Chemistry. Elsevier, 2004, vol. 2004, No 568, p. 65-77. ISSN 0022-0728. info
  • HASOŇ, Stanislav, Simo-Pekka SIMONAHO, Raimo SILVENNOINEN and Vladimír VETTERL. On the adsorption kinetics of phase transients of adenosine at the different carbon electrodes modified with a mercury layer. Electrochimica Acta. UK: Pergamon, 2003, vol. 48, No 1, p. 651-668. ISSN 0013-4686. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Application of carbon electrodes modified with a mercury layer of a different thickness for studies of the adsorption and kinetics of phase transients of cytidine. Journal of Electroanalytical Chemistry. The Netherlands: Elsevier, 2002, vol. 536, No 1, p. 19-35. ISSN 0022-0728. info
  • PALEČEK, Emil, Miroslav FOJTA, František JELEN and Vladimír VETTERL. Electrochemical analysis of nucleic acids. Weinheim: Wiley-VCH Verlag, 2002, 66 pp. Encyclopedia of Electrochemistry, Vol.9, p.365. ISBN 3-527-30401-0. info
  • HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Impedance analysis of DNA and DNA-drug interactions on thin mercury film electrodes. Critical Reviews in Analytical Chemistry. Boca Raton (Florida, USA): CRC Press, 2002, vol. 32, No 2, p. 167-179. ISSN 1040-8347. info
  • HASOŇ, Stanislav, Jakub DVOŘÁK, František JELEN and Vladimír VETTERL. Interaction of DNA with echinomycin at the mercury electrode surface as detected by impedance and chronopotentiometric measurements. Talanta. Elsevier Science, 2002, vol. 56, No 5, p. 905-913. ISSN 0039-9140. info
  • HASOŇ, Stanislav and Vladimír VETTERL. On the formation kinetics of two-dimensional cytidine films. Bioelectrochemistry. The Netherlands: Elsevier, 2002, vol. 57, 1-2, p. 23-32. ISSN 1567-5394. info
  • HASOŇ, Stanislav and Vladimír VETTERL. Two-dimensional condensation of nucleic acid components at mercury film and gold electrodes. Bioelectrochemistry. The Netherlands: Elsevier, 2002, vol. 56, 1-2, p. 43-45. ISSN 1567-5394. info
  • BARD, Allen J. and Larry R. FAULKNER. Electrochemical methods : fundamentals and applications. 2nd ed. New York: John Wiley & Sons, 2001, xxi, 833. ISBN 0471043729. info
  • JELEN, František, Vladimír VETTERL, Petr BĚLUŠA and Stanislav HASOŇ. Adsorptive Stripping Analysis of DNA with Admittance Detection. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2000, vol. 12, No 12, p. 987-992. ISSN 1040-0397. info
  • VETTERL, Vladimír, František JELEN, Viktor DRAŽAN, Luděk STRAŠÁKA and Stanislav HASOŇ. Electrochemical impedance spectroscopy of native and denatured DNA. In DNA Structure and Interaction - Gregor Mendel Symposium, Book of Abstracts. Brno: Biofyzikální ústav AV ČR, 2000, p. 9. info
  • BRETT, Christopher M. A. and Ana Maria Oliviera BRETT. Electroanalysis. Oxford: Oxford University Press, 1998, 88 s. ISBN 0198548168. info
  • BRABEC, Viktor, Vladimír KLEINWÄCHTER and Vladimír VETTERL. Structure, chemical reactivity and electromagnetic properties of nucleic acids. In Bioelectrochemistry: Principles and Practice. Basel, Switzerland: Birkhäuser Verlag, 1997, p. 1-104. 5. ISBN 3-7643-5296-5. info
  • BRABEC, Viktor, Vladimír VETTERL and Oldřich VRÁNA. Electroanalysis of biomacromolecules. In Bioelectrochemistry: Principles and Practice. Basel, Switzerland: Birkhäuser Verlag, 1996, p. 287-359. Bioelectrochemistry:Principles and Practice, Vol.3. ISBN 3-7643-5084-9. info
Teaching methods
lectures, class discussion
Assessment methods
final oral colloquium
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, spring 2012 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2018, recent)
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