PřF:Bi8090 Gene engineering - Course Information
Bi8090 Gene engineering
Faculty of ScienceSpring 2010
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Jiří Doškař, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc. - Timetable
- Thu 11:00–12:50 BR3
- Prerequisites
- Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW ( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biophysics (programme PřF, M-FY)
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Ecotoxicology (programme PřF, M-BI)
- Ecotoxicology (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
- Syllabus
- Definition of gene engineering, its history and perspectives
- Mutagenesis in vitro
- Protein engineering
- Optimalization of gene expression
- Gene cloning in bacteria
- Gene cloning in plants
- gene cloning in animals
- Characteristics of cloning vectors
- Gene transfer in embryonal cells
- Gene therapy
- Preparation of pharmacologically important substances in heterologous systems
- Risc of manipulations with GMO
- Ethical problems
- Literature
- PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
- PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
- Teaching methods
- Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
- Assessment methods
- Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
- Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Spring 2010, recent)
- Permalink: https://is.muni.cz/course/sci/spring2010/Bi8090