Bi5491 Molecular Diagnostics - Practice I

Faculty of Science
Autumn 2009
Extent and Intensity
0/4/0. 4 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
učitelé GMB (seminar tutor)
doc. RNDr. Petr Kuglík, CSc. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Prerequisites
( SOUHLAS || ( B4070 General mikrobiology || B4090 General microbiology || B4090p General microbiology -l || Bi4090c General microbiology - pr. || BX090 || BMB22 General microbiology || B2050 General microbiology || Bi2050c General microbiology pr. )) && ( Bi4035 Molecular biology - lab.pract. || Bi4020c Essential methods in mol. biol )
Basic knowledge of molecular structure a function of procaryotic and eucaryotic genome. Cultiovation techniques.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 0/30, only registered: 0/30
fields of study / plans the course is directly associated with
Course objectives
The subject is a part of basic course Molecular biology for students of discipline Molecular and cellular diagnostic. It comprises the tasks that offer acquiring of basic methods in molecular diagnics.
Syllabus
  • 1. Macrorestriction analysis performed by pulsed-field gel electrophoresis
  • 2. Southern blotting, nonradioactive labeling od DNA.
  • 3. Selective restriction fragment hybridization, estimation of RFLP polymorphisms
  • 4. Applications of polymerase chain reaction (PCR) in diagnostics of bacteria.
  • 5. Protein isolation from animal cells, electrophoresis SDS-PAGE, western blotting, detection of proteins by means antibodies.
  • 6. Estimation of animal cell number in population by means haemocytometry and microscopy.
  • 7.-12. Specialized courses performed in external laboratories, excursions.
Literature
  • Návody k úlohám jsou dostupné u vyučujících
  • PCR Protocols for Emerging Infectious Diseases :a supplement to Diagnostic molecular microbiology : principles and applications. Edited by David H. Persing. Washington: ASM Press, 1996. xii, 180 s. ISBN 1-55581-108-6. info
  • PERSING, D.H., T.F. SMITH, F.C. TENOVER and T.J. WHITE. Diagnostic molecular microbiology. Washington, D.C.: ASM Press, 1993. 642 pp. ISBN 1-55581-056-X. info
  • MILLER, Jeffrey H. A Short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria. New York: Cold Spring Harbor Laboratory Press, 1992. 456 s. ISBN 0879693495. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods
Practical laboratory courses (2 weeks block). The course is completed after work out of protocols and pass a writen test.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2009/Bi5491