The course is offered to students of any study field.
At the end of the course students should understand biochemical and chemical principles of traditional and recent biotechnologies and fundamental processes in fermenters and other apparatuses which provide the biotechnological use of metabolic activity of organisms or enzymes including biochemistry and physiology of organisms applied in biotechnology, selected biotechnological processes (from fermentation products to environment protection), bioprocess kinetics in batch and continuous systems, interpretation of kinetic models in biotechnology and microbial (cell) physiology, and application of immobilised cells and enzymes.
Microbial and enzyme biotechnology, historical survey. Biochemistry, microbiology and engineering principles.
Biochemical and chemical principles of classical and recent biotechnologies. Beer production.
Organic acids, biogas, microbial biomass production as a protein supply, biohydrometallurgy, biotransformation.
Cultivation and production bioreactors, scale-up. Agitation in fermenters, the impact on metabolic activity of organisms.
Sterilization, chemical and physical processes, design criterion for sterilization.
Aeration in bioprocesses. The theory of oxygen transfer.
Methods for determination of the mass-transfer oxygen coefficient. Aeration parameters in bioreactors in relation to oxygen demand by production cultures and enzymes, scale-up.
Batch culture. Growth and production kinetics. Models of substrate utilization and product formation.
Cell growth and death kinetics. Kinetic models in biotechnology and microbial (cell) physiology, the model selection.
Continuous culture. Determination of kinetic and physiological parameters in a chemostat. The relationship with the batch culture.
Immobilised cells and enzymes, principles and applications.
Bioreactors based on immobilised cells and enzymes, kinetic pattern.
KAŠTÁNEK, František. Bioinženýrství. Vyd. 1. Praha: Academia, 2001. 334 s. ISBN 8020007687. info
STANBURY, Peter F., Allan WHITAKER and Stephen J. HALL. Principles of fermentation technology. 2nd ed. Oxford: Pergamon, 1995. xviii, 357. ISBN 0-08-036131-5. info
DORAN, Pauline M. Bioprocess engineering principles. London: Academic Press, 1995. xiv, 439 s. ISBN 0-12-220856-0. info
KRUMPHANZL, Vladimír and Zdeněk ŘEHÁČEK. Mikrobiální technologie : buňka a techniky jejího využití. 1. vyd. Praha: Academia, 1988. 360 s., 24. info
ALEXANDER, Martin. Biodegradation and bioremediation. San Diego: Academic Press, 1994. 302 s. ISBN 0-12-049860-X. info
Lectures on selected parts of biotechnology. Discussions on detailed subjects.
Lectures, discussions during lectures. Oral exam. Understanding of principles and processes is required. Fifty % of correct answers from the part of bioprocess kinetics is required to continue with the exam.
Language of instruction
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.