C6200 Biochemical Methods

Faculty of Science
Spring 2019
Extent and Intensity
4/0/0. 4 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Tue 13:00–14:50 B11/205, Wed 13:00–14:50 B11/205
Prerequisites
( c3181 Biochemistry I && C4182 Biochemistry II )|| C3580 Biochemistry
Basic knowledge of biochemistry, analytical and physical chemistry.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 11 fields of study the course is directly associated with, display
Course objectives
The students should understand at the end of the course the basic methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of enzymes and biopolymers, determination of purity and molecular properties of enzymes, spectroscopic, electrochemical and physical methods of analysis in biochemistry.
Learning outcomes
At the end of this course the students should not only theoretically understand but be able practically apply the methods of disintegration of animal, vegetal and microbial tissues, methods of isolation, purification of proteins, nucleic acids and other biopolymers, methods for determination their purity and molecular properties, spectroscopic, electrochemical and physical methods of analysis in biochemistry. They should be able to apply these knowledges in their scientific research working on bachelor, master and PhD theses and finally during their following employment as well.
Syllabus
  • 1. Introduction, the strategy of treatement of biological material 2. Disintegration, centrifugation, sedimentation analysis 3. Phase separation, precipitation, extraction. membrane separation, Concentration and lyophilisation, water treatment 4. Chromatographic methods, principles (adsorption, ion-exchange), chromatofocusation 5. Reversed phase chromatography, ion pair chromatography, hydrophobic, GPC, affinity, gaz chromatography 6. Electromigration methods, principles, zone electrophoresis 7. IEF, isotachophoresis, blotting 8. Methods of determination of mol. mass and shape of molecules, mass spectrometry, Osmometry, Viscosimetry, translation and rotation diffusion 9. X-ray analysis, electrochemical methods, principles, potentiometry, ISE, biosensors 10. Amperometry, polaropgraphy, voltammetry, calorimetry, biochemical applications. Fast reactions, ion exchange, ligand-polymer interactions, methods of determination binding parameters. 11. Spectrometry. Electron spectra, transitions, ground and excited state, influence of solvents, UV-VIS spectrometry. Use for the study of protein structure 12. Luminiscence methods. Physical parameters, fluorescence quenching, energy transfer, fluorescence polarisation, phosphorimetry, 13. IR spctroscopy, Raman spectroscopy, ORD, CD 14. NMR, EPR, Mossbauer spectroscopy
Literature
  • KALOUS, Vítěz. Metody chemického výzkumu. 1. vyd. Praha: SNTL - Nakladatelství technické literatury, 1987, 430 s. URL info
  • ANZENBACHER, Pavel and Jan KOVÁŘ. Metody chemického výzkumu pro biochemiky. 1. vyd. Praha: Ministerstvo školství ČSR, 1986, 199 s. info
  • KALOUS, Vítěz and Zdeněk PAVLÍČEK. Biofyzikální chemie. 1. vyd. Praha: Nakladatelství technické literatury, 1980, 349 s. info
Teaching methods
Lectures with a demonstration of the taught topic by means of demonstration videos and programs that are available for the students on web page of this course.
Assessment methods
Basic lecture given by Prof. Zbořil and Prof. Glatz, with invited external lectures that are expert in specific fields of biochemical methods. The exam is in the oral form. Examiner Prof. Zbořil. The time for the test is one hour, the grades are A to F on the basis of each question classification.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught each semester.
General note: Vyučují učitelé katedry biochemie.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2019, recent)
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