C6560 Biochemistry - laboratory course

Faculty of Science
Spring 2022
Extent and Intensity
0/0/4. 4 credit(s). Type of Completion: z (credit).
Mgr. Kateřina Dadáková, Ph.D. (seminar tutor)
Mgr. Zdeněk Farka, Ph.D. (seminar tutor)
Guaranteed by
doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Biochemistry - Chemistry Section - Faculty of Science
Prerequisites (in Czech)
C5720 Biochemistry || C3580 Biochemistry || C3181 Biochemistry I
Course Enrolment Limitations
The course is offered to students of any study field.
Course objectives
At the end of the course students should have the basic knowledges of basic properties of compounds important for biochemistry, and methodological approaches in the study and analytical application of enzymes including qualitative and quantitative reactions of carbohydrates, amino acids, proteins and nucleic acids,enzyme kinetics and properties of enzymes.
Learning outcomes
Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work
  • Qualitative and quantitative determination of carbohydrates. Qualitative and quantitative determination of aminoacids. Quantitative determination of proteins. Isolation, analysis and determination of nucleic acids. Separation methods. Determination of enzyme activity. pH effect on enzyme reaction. Enzyme kinetics. Inhibition of enzymes. Enzymatic determination of metabolites. Substrate specifity of enzymes. Immobilization of enzymes. Respiratory chain chain of aerobic organisms. Photosynthesis.
  • Návody ke cvičením - viz Studijní materiály
  • VOET, Donald and Judith G. VOET. Biochemie. Translated by Arnošt Kotyk. 1. vyd. Praha: Victoria Publishing, 1995. S. II-XIV,. ISBN 80-85605-44-9. info
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry. New York: John Wiley & Sons, 1999. 1 sv. ISBN 0471586501. info
Teaching methods
laboratory courses
Assessment methods
The first exercise is devoted to learning about safety in     biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 50% of the questions.
Language of instruction
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
General note: Vyučují učitelé katedry biochemie.
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/C6560