B6490 Molecular Diagnostics

Faculty of Science
Spring 2000
Extent and Intensity
1/0/0. 1 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Roman Pantůček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Roman Pantůček, Ph.D.
Prerequisites (in Czech)
( B4020 Molecular biology || B4030 Molecular biology || B6130 Molecular biology ) && ( B6390 Molecular Methods || NOW( B6400 Methods of molecular biology ))
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Syllabus
  • Basic principles of molecular diagnostics (survey of methods). Analysis of microbial genomic DNA, DNA fingerprinting, use of PFGE. Analysis of genes coding for rRNA, ribotyping, rDNA typing. Analysis of polymorfis in spacer regions, analysis of intergenic repetitive sequences, AP-PCR. Analysis of genes (application of DGGE, SSCP, RFLP, CFLP and AFLP). Immunological methods, MLEE. Application in molecular systematics, construction of phylogenetical trees, analysis of nucleic acids similarity. Molecular diagnostics of bacterial pathogens (Mycobacterium, Borrelia, Mycoplasma, Legionella, E. coli, Helicobacter, enterokoky, stafylokoky aj.) Bacterial pathogenesis, virulence factors and their detection, mechanisms of bacterial resistence to antibiotics and gene detection. Molecular diagnostics of viruses. Molecular diagnostics of fungal and protozoal pathogens.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught: every week.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/spring2000/B6490