Histone post-translational modifications are epigenetic marks that strongly affect numerous processes, including cell cycle and protein interactions. Currently, mass spectrometry analysis represents a preferred approach for histone characterization. The diversity of histones and the complexity of their modifications arising from high contents of reactive amine groups in their amino acid sequences make histone characterization challenging. One of the key steps in sample preparation prior to LC-MS/MS analysis is the chemical derivatization of histones. Even though commonly used derivatization agents improve the chromatographic properties and detection of post-translationally modified histone peptides, they still possess certain limits. Within the frame of the master thesis, the student will test alternative derivatization reagents for histone labeling to improve chromatographic separation of histone peptide forms, facilitate identification and localization of natural acylations, and ensure effective ionization of derivatized peptides. The topic covers chemical derivatization of amine groups, LC-MS/MS analysis of histone peptides, and data processing. The experimental part of the study will be done in the laboratories of Zbyněk Zdráhal Research Group, CEITEC-MU.