Curriculum Vitae

Curriculum vitae

Person Identification

• Jan Havliš, born December 17, 1970 in Brno.

Workplace

• Masaryk University
  : Faculty of Science
  :: National Centre for Biomolecular Research
  ::: Laboratory of functional Genomics and Proteomics
  Kamenice 5, CZ-6250 00 Brno

Employment Position

• Associate professor

Education and Academic Qualifications

• 2013: General Linguistics, B.A., "Construction of quasi-natural language - basic concept", Dept Gen Ling & Baltistics, Fac Art, Masaryk Uni
• 2006: Analytical Chemistry, habilitation, "Bioanalytics - HPLC and MS", Dept Analyt Chem, Fac Sci, Masaryk Uni
• 1998: Physical and Theoretical Chemistry, Ph.D., "Noval study of the redox properties of NAD-coenzymes", Dept Phys Theor Chem, Fac Sci, Masaryk Uni
• 1994: Biochemistry, M.Sc., "Interaction of serum albumin with copper(II) ions", Dept Biochem, Fac Sci, Masaryk Uni
• 1992: Biochemistry, B.Sc., Dept Biochem, Fac Sci, Masaryk Uni

Employment Summary

• November 2011 - till yet: associate professor, CEITEC MU & NCBR Fac Sci, Masaryk Uni
• September 2007 - October 2011: associate professor, Inst Experim Biol, Fac Sci, Masaryk Uni
• May 2006 - August 2007: associate professor, Dept Analyt Chem, Fac Sci, Masaryk Uni
• February 1998 - April 2006: assistant protfessor, Dept Analyt Chem, Fac Sci, Masaryk Uni
• October 1997 - January 1998: reseach assistant, Dept Analyt Chem, Fac Sci, Masaryk Uni

Pedagogical Activities

• 1994-1997: Lab. exercises in physical chemistry
  1997-2007: Methods in chemical research (part HPLC), lab. exercises
  1999-till yet: Separation methods A - cze, lecture
  1999: Petr Marsalek, master's thesis - consultant, finished
  1999: Alice Kotulanova, master's thesis - consultant, finished
  1998: Hana Chroma, PhD thesis - consultant, finished
  1999-2002: Sona Humplikova, master's thesis - supervisor, finished
  1999-2007: Seminar II (for students of Analytical Chemistry)
  2000-2001: Alice Kotulanova, PhD thesis - consultant, unfinished
  2000-till yet: Bioanalytics I - cze, lecture
  2000: Lasers in chemistry, lecture
  2003: Analysis of organic compounds 2 - lecture and lab. exercises
  2003-2005: Roman Tomas, master's thesis - supervisor, finished
  2004-2007: Tomas Prosecky, master's thesis - supervisor, finished
  2004-2005: Hana Ovadová, bachelor's thesis - supervisor, finished
  2005-2007: Michal Horak, master's thesis - consultant, finished
  2005-till yet: Bioanalytics II - cze, lecture
  2005-2007: Hana Ovadova, master's thesis - supervisor, finished
  2006: Lenka Monincova, bachelor's thesis - supervisor, finished
  2006-till yet: Lenka Monincova, master's thesis - supervisor, finished
  2006-till yet: David Potesil, PhD thesis - supervisor, finished
  2007-till yet: Hana Ovadova, PhD thesis - supervisor, unfinished
  2007-till yet: Radek Pilny, PhD thesis - supervisor, unfinished
  2007-till yet: Monika Brodova, PhD thesis - supervisor, unfinished
  2008-till yet: Petra Staskova, bachelor's thesis - supervisor, finished
  2008-till yet: Separation methods B - cze, lecture
  2008-till yet: Discover the science I & II - cze, lectures
  2008-till yet: Bioanalytics I - eng, lecture
  2008-till yet: Bioanalytics II - eng, lecture
  2009-till yet: Separation methods A - eng, lecture
  2009-till yet: Separation methods B - eng, lecture
  2009-2011: Marcela Zupkova, master's thesis - supervisor, finished
  2009-2011: Jan Mlatecek, master's thesis - consultant, finished
  2009-2011: Petr Rasovsky, master's thesis - consultant, finished
  2011-2012: Kamil Mikulasek, bachelor's thesis - consultant, finished
  2012-till yet: Seminary BGP I
  2012-till yet: Proteomics - cze (parts Intro, Proteins & Peptides, Expression Proteomics), lecture
  2012-2014: Kamil Mikulasek, master's thesis - consultant, finished
  2013-till yet: Seminary BGP II
  2013-till yet: Seminary BGP III
  2013-till yet: Methods in genomics and proteomics - cze (part Separation of Proteins), lecture
  2013-2015: Tomas Rozman, master's thesis - consultant, finished
  2014-till yet: Seminary BGP IV
  2014-till yet: Constructed languages & the phenomenon of language construction - cze, lecture
  2014-till yet: Law, ethics and philosophy of science (parts Philosophy of Science, Ethics in Science)- eng, lecture
  2014-till yet: Kamil Mikulasek, PhD thesis - supervisor
  2014-2015: Jan Fiala, bachelor's thesis - consultant, finished
  2015-till yet: Introduction to interlinguistics - cze, lecture
  2015-2017: Jan Fiala, master's thesis - consultant, finished
  2016-till yet: Proteomics - eng (parts Intro, Proteins & Peptides, Expression Proteomics), lecture
  2016-till yet: Discover the science I & II - eng, lectures
  2017-till yet: Methods in genomics and proteomics - eng (part Separation of Proteins), lecture
  

Scientific and Research Activities

• 1990-1994: Interaction of serum albumin with metal ions (Cu, Ni, Zn, Fe)
  1994-1998: Study of the redox properties of NAD-coenzymes
  1998-2007: MALDI TOF MS - quantification
  1998-2003: Determination of purins and pyrimidins by means of MALDI TOF MS in biol. samples as markers of gen. diseases
  1999-2001: Demethylation state of DNA within FRM1 gene
  2001-2003: Analyses of important substrates and metabolites of some diaminooxidases
  2001-2011: Study of protein complexes; Metalloproteins
  2001-till yet: Quantification of proteins and peptides using MS
  2003-till yet: Constructed languages and interlinguistics
  2010-till yet: QSRR of ultra short oligonucleotides in II-RPLC & HILIC
  

Internship and stays for the purpose of study or work

• February 1998: University of Vienna (Universitaet Wien), Faculty of Natural Sciences and Mathematics, Department of Analytical Chemistry, Bio Mass Spec, Austria, Dr Guenter Allmaier
  April 2001: University of Szeged (Szegedi Tudomanyegyetem), Faculty of Science, Department of Inorganic and Analytical Chemistry, Hungary, Dr Antal Peter
  2001-2003: Max Planck Institute, Molecular Cell Biology and Genetics, Mass Spectrometry Group, Dresden, Germany, Dr Andrej Shevchenko
  
• 2001/10/01 – 2003/09/29: Max-Planck-Institut für Molekulare Zellbiologie und Genetik, DEU
  • dvouletý postdoktorální pobyt zaměřený na výzkum v oblasti proteomiky

University Activities

• member of Doctoral Board in Life Sciences
  member Field Committee Genomics and Proteomics
  chair of of Field Committee Bio-omics
  since 2017 - senator of the AS of Fac Sci, Masaryk Uni
  since 2018 - deputy for pedagogical matters, NCBR

Extrauniversity Activities

• 2006-2011: secretary of Czech Chemical Society (CSCH), branch Brno
  2010-2015: member of Executive Board of Czech Society for Mass Spectrometry
  

Selected Publications

• MIKULÁŠEK, Kamil, Kamil JAROŇ, Petr KULHÁNEK, Miroslava BITTOVÁ and Jan HAVLIŠ. Sequence-dependent separation of trinucleotides by ion-interaction reversed-phase liquid chromatography A structure-retention study assisted by soft-modelling and molecular dynamics. Journal of Chromatography A. Amsterdam: Elsevier Science BV, 2016, vol. 1469, October, p. 88-95. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2016.09.060. supplementary data info
• HAVLIŠ, Jan, Tatiana ŠTOSOVÁ, Marek ŠEBELA, Pavel ŘEHULKA, Ondrej ŠEDO and Zbyněk ZDRÁHAL. Evaluation of the possible proteomic application of trypsin from Streptomyces griseus. Analytical Biochemistry. San Diego: Academic Press, 2008, vol. 376, No 1, p. 94-102. ISSN 0003-2697. info
• HAVLIŠ, Jan and Mathias MANN. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nature Protocols. U.S.A.: Nature Publishing Group, 2008, vol. 1, No 6, p. 2856-2860. ISSN 1745-2473. info
• HAVLIŠ, Jan and Christine WALCH-SOLIMENA. Nucleo-cytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Molecular and Cellular Biology. Washington, D.C.: ASM, 2008, vol. 19, No 3, p. 1046-61, 16 pp. ISSN 0270-7306. info
• POTĚŠIL, David, Soňa ČEJKOVÁ, Ludmila ROČŇOVÁ, Martin TRBUŠEK, Zbyněk ZDRÁHAL and Jan HAVLIŠ. Sensitization of B-CLL lymphocytes, quest for a protein marker. Which mass spectrometry quantitative technique will give answer? 2008. URL URL info
• HAVLIŠ, Jan. Speciace metaloproteinů - využití paralelně kombinovaných spektrometrických technik (Metalloprotein speciation - use of parallel combined spectrometric techniques). In 13. Spektrometrická konference - sborník. 2007. info
• HAVLIŠ, Jan and Hana OVADOVÁ. METALO + PROTEIN = GE + LA + ICP + MS. In KANICKÝ, Viktor and Tomáš VACULOVIČ. 13. Spektrometrická konference - sborník. 2007. info
• HAVLIŠ, Jan. Analýza metaloproteinů pomocí MS - paralelně kombinované techniky GLIM (Analysis of metalloproteins using MS - parallel combined techniques GLIM). In 8. ročník Školy hmotnostní spektrometrie - Sborník přednášek. Brno: Fakulta Chemická, VUT, Brno, 2007, p. 100-108. ISBN 978-80-214-3477-6. info
• HAVLIŠ, Jan and Lenka MONINCOVÁ. Prediction of tryptic peptide retention times by means of soft modelling as a tool for liquid chromatography-mass spectrometry driven proteomics. Trends in Chromatography. Indie: Research Trends (P) Ltd., 2007, vol. 3, -, p. 65-72. ISSN 0972-8635. info
• HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS, Zbyněk ZDRÁHAL and Andrej SHEVCHENKO. Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation. Proteomics. Weinheim: Wiley-VCH, 2006, vol. 10, No 6, p. 2959-2963, 4 pp. ISSN 1615-9853. info
• HAVLIŠ, Jan, Zbyněk ZDRÁHAL and Ondrej ŠEDO. Protein Modification Degree - An Indirect MS Quantitation Approach. In Abstract Book 17th IMSC Prague. 2006. info
• HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Petr TARKOWSKI, Natalie WIELSCH, Henrik THOMAS and Andrej SHEVCHENKO. Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process. In Abstract Book 17th IMSC Prague. 2006. info
• ŠEBELA, Marek, David KOPEČNÝ, Zbyněk LAMPLOT, Jan HAVLIŠ, Henrik THOMAS and Andrej SHEVCHENKO. Thermostable -cyclodextrin conjugates of two similar plant amine oxidases and their properties. Biotechnology and Applied Biochemistry. 2005, vol. 41, p. 77-84, 7 pp. ISSN 0885-4513. info
• HAVLIŠ, Jan, Tobias OELSCHLAEGEL, Martin SCHWICKART, Joao MATOS, Aliona BOGDANOVA, Alain CAMASSES, Andrej SHEVCHENKO and Wolfgang ZACHARIAE. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. United States of America: Cell Press, Cambridge, MA, USA, 2005, vol. 120, No 6, p. 773-788, 15 pp. ISSN 0092-8674. info
• HAVLIŠ, Jan, Ondrej ŠEDO and Zbyněk ZDRÁHAL. Indirect quantification of protein modification degree. In Book of abstracts 2nd Czech Proteomic Conference. 2005. info
• HAVLIŠ, Jan. Praktické aspekty absolutní kvantifikace proteinů a peptidů pomocí MS (Practical aspects of absolute quantification of proteins and peptides using MS). In 3. ročník Školy HPLC/MS - Sborník přednášek. Pardubice: Univerzita Pardubice, 2005, p. 175-179, 4 pp. ISBN 80-7194-785-7. info
• HAVLIŠ, Jan. Využití izotopového značení v hmotnostní spektrometrii (Use of isotope labelling in mass spectrometry). In 3. ročník Školy HPLC/MS - Sborník přednášek. Pardubice: Univerzita Pardubice, 2005, p. 147-153, 6 pp. ISBN 80-7194-785-7. info
• HAVLIŠ, Jan, Klára NOVOTNÁ and Josef HAVEL. Optimization of high performance liquid chromatography separation of neuroprotective peptides. Fractional experimental design combined with Artificial neural networks. Journal of Chromatography A. Elsevier B.V., 2005, vol. 1096, 1-2, p. 50-57. ISSN 0021-9673. info
• HAVLIŠ, Jan, Tatiana ŠTOSOVÁ, Marek ŠEBELA and René LENOBL. Proteolytické enzymy: význam pro proteomiku (Proteolytic enzymes: Significance for proteomics). Chemické listy. Praha: Czech Chemical Society, 2005, vol. 99, No 12, p. 896-905. ISSN 0009-2770. info
• THOMAS, Henrik, Jan HAVLIŠ, Jan PEYCHL and Andrej SHEVCHENKO. Dried-Droplet Probe Preparation on AnchorChip(TM) Targets for Navigating the Acquisition of MALDI TOF Spectra by Fluorescence of Matrix / Analyte Crystals. Rapid Communications in Mass Spectrometry. United Kingdom: John Wiley & Sons, Ltd, 2004, vol. 18, No 9, p. 923-930. ISSN 0951-4198. info
• SCHWICKART, Martin, Jan HAVLIŠ, Bianca HABERMANN, Aliona BOGDANOVA, Alain CAMASSES, Tobias OELSCHLAEGEL, Andrej SHEVCHENKO and Wolfgang ZACHARIAE. Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27. Molecular and Cellular Biology. Washington, D.C.: ASM, 2004, vol. 24, No 8, p. 3562-3576. ISSN 0270-7306. info
• HAVLIŠ, Jan and Andrej SHEVCHENKO. Absolute Quantification of Proteins in Solutions and in Polyacrylamide Gels by Mass Spectrometry. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2004, vol. 76, No 11, p. 3029-3036, 7 pp. ISSN 0003-2700. info
• ŠEBELA, Marek, Zbyněk LAMPLOT, Michal MALOŇ, René LENOBEL, Karel LEMR, Jan HAVLIŠ, Pavel PEČ, Chunhua QIAO and Lawrence M. SAYRE. 1,5-Diamino-2-pentyne is both a substrate and inactivator of plant copper amine oxidases. European Journal of Biochemistry. 2004, No 271, p. 4696-4708, 12 pp. ISSN 0014-2956. info
• HAVLIŠ, Jan, Henrik THOMAS, Marek ŠEBELA and Andrej SHEVCHENKO. Fast Response Proteomics by Accelerated In-gel Digestion of Proteins. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2003, vol. 75, No 6, p. 1300-1306, 6 pp. ISSN 0003-2700. info
• HAVLIŠ, Jan and Martin TRBUŠEK. 5-methyl-cytosine as a marker for monitoring of DNA methylation. Journal of Chromatography B. Amsterdam: Elsevier, 2002, vol. 781, 1-2, p. 373-391. ISSN 1570-0232. info
• HAVLIŠ, Jan, Anna SHEVCHENKO and Andrej SHEVCHENKO. Protein Identification by MALDI TOF MS: Practical Assessment of the Fast In-gel Digestion Protocol. 50th ASMS, Orlando, Florida, USA, 2002. info
• SHEVCHENKO, Anna, Daniel SCHAFT, Assen ROGUEV, Pim PIJNAPPEL, Jan HAVLIŠ, Francis STEWART and Andrej SHEVCHENKO. SEAMing Proteins from the TAP: Charting Protein Interaction Networks in Yeast by Tandem Affinity Purification and Mass Spectrometry. 50th ASMS, Orlando, Florida, USA, 2002. info
• HAVLIŠ, Jan, John Edward MADDEN, Alma Luísa REVILLA VÁZQUEZ and Josef HAVEL. High Performance Liquid Chromatographic determination of deoxycytidine monophosphate and methyl-deoxycytidine monophosphate for DNA demethylation monitoring: Experimental Design and Artificial Neural Networks optimisation. J. Chromatogr. B. Amsterdam: Elsevier Science, 2001, vol. 2001, No 755, p. 185-193. ISSN 0378-4347. info
• CHROMÁ-KEULL, Hana, Jan HAVLIŠ and Josef HAVEL. Reactive Dye Ostazine Black V-B: Determination in Dye-bath and Hydrolysis Montoring by Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry (Reactive Dye Ostazine Black V-B: Determination in Dye-bath and Hydrolysis Monitoring by Matrix Assisted Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry). Rapid Communications in Mass Spectrometry. United Kingdom: John Wiley & Sons, Ltd, 2000, vol. 2000, No 14, p. 40-42. ISSN 0951-4198. info
• ŠEBELA, Marek, Pavel PEČ, Petr GALUSZKA and Jan HAVLIŠ. Characterisation of a homogenous plant aminoaldehyde dehydrogenase. Biochimica et Biophysica Acta. Amsterdam: Elsevier Science B.V., 2000, -, No 1480, p. 329-669. ISSN 0005-2728. info
• CHROMÁ-KEULL, Hana, Jan HAVLIŠ and Josef HAVEL. : Reactive Dye Ostazine Black V-B: Determination in Dye-bath and Hydrolysis Monitoring by Matrix Assisted Laser Desorption-Ionisation Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 1999, vol. 13, No 1, p. 40-43. ISSN 0951-4198. info
• HAVLIŠ, Jan, Alma Luísa REVILLA VÁZQUEZ, Libor KOZÁK and Josef HAVEL. Determination of purins, pyrimidines and their nucleosides in urine by HPLC and MALDI-TOF mass spectrometry. 23rd HPLC, Granada, Spain: n / a, 1999. n / a. info
• HAVLIŠ, Jan and Oldřich JANICZEK. Influence of protein-bound copper(II) on properties of bovine serum albumin-tryptophan complex. Scripta Facultatis Scientiarum Naturalium Universitatis Masarykianae - Chemistry. Brno: Přírodovědecká fakulta MU, 1998, 1997-1998, 27-28, p. 43-49. ISSN 2100-6828. info
• HAVLIŠ, Jan and Marie STUDNICKOVA. Zinc centres in alcohol dehydrogenase from liver and from bakers yeast are metal dithiolenes. Biochemistry and bioenergeics : section of Journal of electroanalytical chemistry and electrochemistry. Lausanne: Elsevier Sequoia S.A., 1997, vol. 43, -, p. 157-159. ISSN 0302-4598. info
• HAVLIŠ, Jan, Oldřich JANICZEK and Martin MANDL. Influence of protein-bound copper(II) on properties of bovine serum albumin-tryptophan complex. In International Biometals Symposium. Calgary: University of Calgary, 1997, p. 25. info

2018/10/29