VSHE0221c Histology and Embryology I - practice

Faculty of Medicine
Spring 2017
Extent and Intensity
0/3/0. 5 credit(s). Type of Completion: z (credit).
doc. MVDr. Martin Anger, CSc. (seminar tutor)
MUDr. Ivana Baltasová (seminar tutor)
prof. MUDr. RNDr. Svatopluk Čech, DrSc. (seminar tutor)
MUDr. Jana Dumková, Ph.D. (seminar tutor)
doc. MVDr. Aleš Hampl, CSc. (seminar tutor)
Mgr. Hana Kotasová, Ph.D. (seminar tutor)
doc. RNDr. Irena Koutná, Ph.D. (seminar tutor)
MUDr. Irena Lauschová, Ph.D. (seminar tutor)
MVDr. Anna Mac Gillavry Danylevska, Ph.D. (seminar tutor)
MUDr. Eva Mecová (seminar tutor)
doc. MUDr. Miroslava Sedláčková, CSc. (seminar tutor)
MUDr. Veronika Sedláková, Ph.D. (seminar tutor)
doc. RNDr. Petr Vaňhara, Ph.D. (seminar tutor)
Guaranteed by
doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology - Theoretical Departments - Faculty of Medicine
Contact Person: doc. MUDr. Miroslava Sedláčková, CSc.
Supplier department: Department of Histology and Embryology - Theoretical Departments - Faculty of Medicine
Prerequisites (in Czech)
VSAN0131s Anatomy I s && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue.
3. Identify tissue types in histologic slides.
4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs.
5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis.
6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord.
7. Characterize stages of embryonic development.
  • 1. Introduction, organization of practicals, teaching aids. Overview of histological methods. Tissue processing for light and electron microscopy - explanation and film. Basic histochemistry and immunocytochemistry.
  • 2. Cytology: Ultrastructure of the cell nucleus during interphase. Ultrastructure of cell organelles I (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes).
  • 3. Ultrastructure of cell organelles II (peroxisomes, and the centriol). Cell inclusions. The arrangement of cell surfaces: apical, lateral, and basal ones. Intercellular junctions: adhering, occluding, and communicating ones.
  • 4. General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin–eosine-saffron /HES/ (esophagus), AZAN (prostate), impregnation (cerebellum). Connective tissue proper - classification and examples. Slides: Funiculus umbilicalis, Esophagus, Posterior segment of the eye, Lien, Aorta.
  • 5. Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, Auricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification.
  • 6. Epithelial tissue: epithelial membranes (covering epithelia) and glandular epithelium. Slides: Ren, Vesica fellea, Trachea, Esophagus, Ureter, Palpebra, Skin from the finger tip. Intestinum tenue, Intestinum crassum, Glandula parotis, Glandula submandibularis.
  • 7. Muscle tissue. Slides: Apex linguae, Cardiac muscle tissue Intestinum tenue. Nerve tissue. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
  • 8. Revision practical: a skill test of practical diagnosis of the connective and supporting, epithelial, muscle and nerve tissues by use of the microscope and videocamera under supervision of tutors. Videorecords.
  • 9. Microscopic anatomy. Microscopic structure of the heart and blood vessels. Slides: Artery of the muscular type with a vein, Aorta, Vena cava, Myocardium.
  • 10. Blood cells: Preparation of blood smears and their staining. Cytology of formed elements of blood. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Slide: A smear of peripheral blood stained by Pappenheim's method.
  • 11. Hematopoiesis: Erythropoiesis, granulopoiesis and development of thrombocytes. Development of lymphocytes and monocytes. Microscopic structure of the bone marrow. Slide: A bone marrow smear stained by Pappenheim's method.
  • 12. Microscopic structure of the lymphatic system. Slides: Lymphonodus, Lien (spleen), Thymus of a young individual, Thymus of the adult (with involution changes), Palatine tonsil, Lingual tonsil.
  • 13. Microscopic structure of the respiratory system. Slides: Nasal conchae, Epiglottis, Larynx, Trachea, Pulmo. Credits.
    recommended literature
  • ČECH, Svatopluk, Drahomír HORKÝ, Irena LAUSCHOVÁ, Miroslava SEDLÁČKOVÁ and Jitka ŠŤASTNÁ. Histologická praktika a metody vyšetřování tkání a orgánů (Histological practicals and methods of investigation of tissues and organs). 1.dotisk 1. vydání. Brno: Vydavatelství MU Brno-Kraví hora, 2002. 162 pp. ISBN 80-210-1774-0. info
    not specified
  • MARTÍNEK, Jindřich and Zdeněk VACEK. Histologický atlas. 1. vyd. Praha: Grada, 2009. 134 s. ISBN 9788024723938. info
  • EROSCHENKO, Victor P. Di Fiore's atlas of histology with functional correlations. 9th ed. Philadelphia: Lippincott Williams-Wilkins, 2000. xv, 363. ISBN 0683307495. info
Teaching methods
Content of this course includes study of slides and electron micrographs in microscopic hall as well as class discussion on the base of materials in the text book.
Assessment methods
Testing of knowledge: Student must prove sufficient level of knowledge by written test examination. Each student completes 5 partial tests during semester. Tests are evaluated by point for correct answer. More than half number of correct answers (points) is evaluated as "YES". All of these tests must be successful. In case of failure, only 1 resit is possible. There is condition 5 from 5 (ie. 5 YES / 5 regular tests) or 5 from 6 (ie. 4 YES / 5 regular tests + 1 YES / 1 resit). If student does not fulfill this condition, credit test follows in the relevant exam period. This test covers all topics studied during semester. In case of failure in credit test, credit will not be given and student must enroll the course again.
Conditions for obtaining credit: 1. Attendance at all practical exercises (100% participation, all absences must be regularly excused (in IS) and substituted).
2. Successful completion of all tests.
3. Submission of all protocols (correctly completed forms of protocols signed by teacher).
Language of instruction
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016.
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