PřF:FB820 Struct. electron microscopy - Course Information
FB820 Structural electron microscopy
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
- Teacher(s)
- Ing. Tibor Füzik, Ph.D. (lecturer)
Mgr. Jiří Nováček, Ph.D. (lecturer)
Ing. Ondřej Sháněl, Ph.D. (lecturer), Ing. Tibor Füzik, Ph.D. (deputy) - Guaranteed by
- Mgr. Jiří Nováček, Ph.D.
Department of Condensed Matter Physics – Physics Section – Faculty of Science
Supplier department: Department of Condensed Matter Physics – Physics Section – Faculty of Science - Timetable
- Wed 13:00–14:50 Fs1 6/1017
- Prerequisites
- Knowledge of English, mathematics, and physics at the grammar school level.
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- The course will provide an introduction to electron microscopy for structural, cell biology. Students will gain practical knowledge oriented towards understanding sample preparation, principles of electron microscope function, measurement methods and data analysis.
- Learning outcomes
- Upon completion of the course, students will gain:
- an understanding of the principles of the electron microscope
- understanding of the principles of image formation in electron microscopy
- ability to evaluate the suitability of electron microscopy for problem solving in biological research
- an understanding of the methods of data analysis - Syllabus
- 1. Interaction of electron with a matter
- 2. Anatomy of electron microscope
- 3. Principles of image formation
- 4. Sample preparation for life science electron microscopy
- 5. Fourier transform
- 6. 3DEM methods
- 7. 3DEM methods – single particle analysis
- 8. 3DEM methods – electron tomography
- 9. Data interpretation
- Literature
- recommended literature
- Annie Cavalier, Daniele Spehner, Bruno M. Humbel: Handbook of Cryo-Preparation Methods for Electron Microscopy (Methods in Visualization), CRC Press; 1st Ed., 2008
- R. A Crowther: The Resolution Revolution: Recent Advances In cryoEM, Volume 579 (Methods in Enzymology) 1st Edition, Academic Press, 2016
- John Kuo: Electron Microscopy: Methods and Protocols (Methods in Molecular Biology), Humana Press; 3rd ed. 2014
- Teaching methods
- Lectures, class discussions
- Assessment methods
- Semester work, written examination
- Language of instruction
- Czech
- Follow-Up Courses
- Further Comments
- Study Materials
The course is taught annually.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/FB820