PřF:C7187 Experimental oncology - Course Information
C7187 Experimental oncologyFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- doc. Mgr. Pavel Bouchal, Ph.D. (lecturer)
doc. Mgr. Roman Hrstka, Ph.D. (lecturer)
MUDr. Petr Müller, Ph.D. (lecturer)
MUDr. Rudolf Nenutil, CSc. (lecturer)
RNDr. Bořivoj Vojtěšek, DrSc. (lecturer)
- prof. RNDr. Zdeněk Glatz, CSc.
Department of Biochemistry - Chemistry Section - Faculty of Science
- Mon 15:00–16:50 A9-316
- C4182 Biochemistry II || Bi4020 Molecular biology
This course is suitable for students of master, doctoral and of 3rd year of bachelor study in biochemistry and molecular biology. The knowledge at biochemisty and molecular biology basic courses level is required.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- Fields of study the course is directly associated with
- there are 15 fields of study the course is directly associated with, display
- Course objectives
- At the end of the course, the students will understand the basics of biology and biochemistry of tumor cells as well as the mechanisms of malign transformation and spreading of tumors. They should be able to apply genomics and proteomics approaches and to use the main database sources in experimental oncology. The students should be able to explain the approaches used in production and purification of proteins and antibodies, principles of moleclar cloning, gene therapy and the use of therapeutical antibodies. They should be able to make decisions about the proper use of biological material like model systems (cell lines), human tissues, or human plasma. The lecturers will provide insights into oncological pharmacology including drug discovery and testing, and into the basics of the use of viruses in experimental oncology. They will focus one of the lessons on the aspects of cooperation and carrier in science in general.
- 1. [26.10.] Introduction, organization of the course (Dr. Bouchal). Cooperation and carrier in science (Dr. Vojtěšek). Chemical, biological and physical factors of carcinogenesis (Dr. Bouchal). Chemical carcionogens, Ames' test. Biochemical characteristics of tumors.
- 2. [2.11.] Tumor cell biology I (Dr. Hrstka). Mechanisms of malign transformation. Angiogenesis, formation of metastases. Model systems in applied research. Model organisms. Applied biology of the cell. Tumor cell biology II (Dr. Hrstka). Protein p53, the use as predictor, potential use during the therapy. Therapy focused on the cell cycle.
- 3. [9.11.] Genomic approaches in experimental oncology (Dr. Hrstka). PCR based methods, chip based methods. Gene engineering.
- 4. [16.11.] Proteomic approaches in experimental oncology (Dr. Bouchal). Protein and peptide separation approaches. Mass spectrometry for protein identification and quantitation. Protein database sources and algorithms: ExPASy, Human Protein atlas. Clinical proteomics studies: Design, statistical evaluation, data interpretation. Biomarkers.
- 5. [23.11.] Gene therapy (Dr. Müller). Diseases curable by gene therapy. Vectors for gene therapy. Perspectives of gene therapy. Viruses and their use in experimental oncology. Characterization and categories of viruses. Tumors and viruses. Adenovirus and lentivirus vectors. Viruses and gene therapy. Protein engineering (Dr. Müller). Protein-protein interactions, „phage display“.
- 6. [30.11.] Protein and expression and purification (Dr. Vojtěšek). The use of expression systems for protein expression. The use of „Tag sequences“. Protein preparation for production of monoclonal antibodies. Protein preparation for functional studies. Design, production and characterization of antibodies (Dr. Vojtěšek). Immunization schema, suitable animals and antigens. Production, purification, characterization and labeling of monoclonal and polyclonal antibodies. Application and humanization of antibodies.
- 7. [7.12.] The use of human biological material in research (Dr. Nenutil). Human tissues as a source of information and a tool for validation of data obtained from experimental models. The use and processing of tissue samples. Predictive and prognostic pathology. Biological characteristics of tumors and their prognosis. Biological predictors of therapeutical response.
- 8. [14.12.] Drug development (Dr. Müller). Therapeutical substance, pharmacodynamics and pharmacokinetics. The models used for „drug screening“. Databases of chemical substances versus natural products. Quantitative and structural analysis of the potential therapeutical substance. Rational drug development. Clinical studies.
- REJTHAR, Aleš and Bořivoj VOJTĚŠEK. Obecná patologie nádorového růstu. 1. vyd. Praha: Grada, 2002. 206 s. ISBN 802470238X. info
- Teaching methods
- Presentations by professionals followed by class discussion and answers to students' questions. Practical use of database sources.
- Assessment methods
- Written exam followed by a short discussion with the lecturer.
- Language of instruction
- Further comments (probably available only in Czech)
- Study Materials