C7176 DNA diagnostics

Faculty of Science
Autumn 2011
Extent and Intensity
0/4/0. 4 credit(s). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Omar Šerý, Ph.D. (lecturer), doc. Mgr. Jan Lochman, Ph.D. (deputy)
Mgr. Lukáš Častulík (seminar tutor)
Guaranteed by
prof. RNDr. Omar Šerý, Ph.D.
Department of Biochemistry - Chemistry Section - Faculty of Science
Timetable of Seminar Groups
C7176/01: Tue 9:00–12:50 C05/108
C7176/02: Wed 14:00–17:50 C05/108
C7176/03: Thu 11:00–14:50 C05/108
C7176/04: Fri 9:00–12:50 C05/108
Prerequisites
C7175 DNA diagnostics
It is necessary to absolve course DNA diagnostics.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 64 student(s).
Current registration and enrolment status: enrolled: 0/64, only registered: 0/64, only registered with preference (fields directly associated with the programme): 0/64
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to perform the practical DNA analysis - isolate DNA by different methods from different materials, use different PCR methods, restriction analysis, electrophoresis on agarose gel, DNA sequencing, and the usage of immunochromatographic tests.
Syllabus
  • 1. The opening theoretical preparation, lab safety instructions and explanation of DNA isolation methods from buccal mucosa. 2. The detection of the I/D polymorphism of ACE gene using PCR and restriction analysis and detection of the Taq I A polymorphism of DRD2 gene (I). 3. The detection of the I/D polymorphism of ACE gene using PCR and restriction analysis and detection of the Taq I A polymorphism of DRD2 gene (II). 4. The isolation of DNA from a tick and the detection of B.b. sensu lato using RealTime PCR and nested PCR. 5. Topic boreliosis: nested PCR, the detection of B.b. sensu lato, the agarose electrophoresis of nested PCR products, sequencing analysis. 6. The assessment of DNA sequencing results from part of a genome B.b. sensu lato and the classification into species, design of primers and probes. 7. The detection of thrombophilic factors via fragmentation analysis on genetic analyzer. 8. The DNA sequencing of part of the gene for lactase - the determination of lactose intolerance. 9. The comparison of two “housekeeping” genes in order to establish the relative quantity of RNA from human tissue. 10. The assessment of drug metabolites and other substances by using of immunochromatographic tests.
Literature
  • Manual of commercial methods in clinical microbiology. Edited by Allan L. Truant. Washington: ASM Press, 2002. xix, 481 s. ISBN 1-55581-189-2. info
  • The nucleic acid protocols handbook. Edited by Ralph Rapley. Totowa, N.J.: Humana Press, 1999. xxii, 1050. ISBN 0896038416. info
Teaching methods
2-3 lectures with theoretical preparation and laboratory work.
Assessment methods
Graded credit according obtained laboratory protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Nutností zápisu do předmětu je úspěšné absolvování zkoušky z DNA diagnostiky.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2011/C7176