Bi7090 Molecular biology of eukaryotes

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Taught in person.
prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Thu 12:00–13:50 B11/335
( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6401 Bachelor Thesis II || Bi6491 Bachelor Thesis LGMD II || Bi6122 Bachelor thesis HB II || Bi1041 Introd. to Comp. Biology I || Bi6005 Bc. thesis II || Bi6006 Bc. thesis anim. phys. II || Bi6007 Bc. thesis || SOUHLAS )
Essential molecular biology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
Learning outcomes
At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
  • 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
    recommended literature
  • ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008. xxxiii, 12. ISBN 9780815341062. info
Teaching methods
Lectures including brief discussions with students.
Assessment methods
Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
Language of instruction
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020.
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