PřF:Bi7090 Eukaryotic cells - Course Information
Bi7090 Molecular biology of eukaryotesFaculty of Science
- Extent and Intensity
- 2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Taught in person.
- prof. RNDr. Jan Šmarda, CSc. (lecturer)
prof. RNDr. Jana Šmardová, CSc. (lecturer)
- Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: prof. RNDr. Jan Šmarda, CSc.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Thu 12:00–13:50 B11/335
- ( Bi4010 Essential molecular biology || Bi4020 Molecular biology ) && ( Bi6401 Bachelor Thesis II || Bi6491 Bachelor Thesis LGMD II || Bi6122 Bachelor thesis HB II || Bi1041 Introd. to Comp. Biology I || Bi6005 Bc. thesis II || Bi6006 Bc. thesis anim. phys. II || Bi6007 Bc. thesis || SOUHLAS )
Essential molecular biology.
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Bioanalytical Laboratory Diagnostics in Medicine - Medical Genetics and Molecular Diagnostics (programme PřF, N-LGM)
- Human Biology (programme PřF, N-BCL)
- Medical Genetics and Molecular Diagnostics (programme PřF, N-BI)
- Molecular Biology and Genetics (programme PřF, N-EXB, specialization Antropogenetika)
- Molecular Biology and Genetics (programme PřF, N-MBG)
- Course objectives
- The course aims to overview the molecular basis of various processes and pathways in eukaryotic cells; such overview serves as basis to understand general principles controling functions of cells in multicellular organisms.
- Learning outcomes
- At the end of the course students will acquire general knowledge on recent developments within the field of molecular biology of eukaryotic cell. He/she will understand molecular mechanisms of cell cycle and principles of its regulation, structure of DNA in chromatin, principles of signal transduction and programmed cell death. Using this knowledge, he/she should describe and discuss mechanisms of cancerogenesis be able to delineate the major differences between healthy and cancer cells. In addition, students will understand the ways how eukaryotic cells communicate with neighbour cells and extracellular matrix and principles of protein folding, traficking and degradation.
- 1. Intracellular compartments and protein traficking: principloes of protein sorting, signal sequences, the role of endoplasmic retikulum, protein folding, chaperones, Golgi apparatus, vesicular transport, phagocytosis 2. Cell cytoskeleton: microtubules, actine filaments, intermediate filaments,nuclear skeleton 3. Extracellular matrix: cell wall, cellulose synthesis, glykocalyx, matrix components, collagens, elastin, fibrilin, laminin, elasctic fibres, proteoglykans, glykoproteins, fibrinogen 4. Cell cycle: molecular principles of regulation, checkpoints, methods if analysis, cyclins and CDKs, deregulation of cancer 5. Cell-cell and cell-extracellular matrix interactions: matrix types, structure, function, kolagen, hyaluronic acid, proteoglykans, cadherins, laminins, fibronectin, selectins, integrins, types of cell-cell interactions 6. Molecular base of neuromuscular system (neural cells, synapses, structure of transmembrane channel systems, neuro-smuscular connections, thin and thick filaments, molecular base of muscle contraction, differentiation of muscle cells in vitro, MyoD protein. 7. Cell signaling: ligands, signaling pathways, receptors, SH2 domains, secondary messengers, JAK/STAT, MAP kinases, Ras protein, effectors, G proteins, cAMP, Ca2+ ions in signaling, PKA, PKC, PKCa 8. Chromatin: nucleosomes, higher levels of chromatin structure, changes in chromatin – functional implications, methods of chromatin analysis 9. Protein degradation in cells: lysosomes, autophagy, ubiquitin, proteasome – structure and function, diseases resulting from failure of the proteasome. 10. Molecular base of cancerogenesis: attributes of tumor cells, base of malignant transformation, the roles of oncogenes, tumor suppressors and cell death regulators, protooncogenes and their protein products, oncogene cooperation in malignant transformation, apoptosis, the role of viruses in cancerogenesis. 11. Mechanisms of cell death: apoptosis, inducers of cell death, markers, molecules driving apoptosis, caspases, intrinsic and extrinsic pathways
- recommended literature
- ALBERTS, Bruce. Molecular biology of the cell. 5th ed. New York, N.Y.: Garland science, 2008. xxxiii, 12. ISBN 9780815341062. info
- Teaching methods
- Lectures including brief discussions with students.
- Assessment methods
- Examination is based on written test and/or oral interview. At least 50% of correct answers are required to pass the test. During the interview, students are expected to answer 1 - 2 questions of examinator. The questions deal with issues described during the course.
- Language of instruction
- Follow-Up Courses
- Further comments (probably available only in Czech)
- Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
- Listed among pre-requisites of other courses
- Teacher's information
Requirements for succesfull passing the exam: understanding of principles driving key biological processes in eukaryotic cells, including molecules involved in these processes.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2021/Bi7090