PřF:Bi8120 Animal Cell Biology - Course Information
Bi8120 Animal Cell Biology
Faculty of ScienceSpring 2010
- Extent and Intensity
- 2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- Teacher(s)
- prof. RNDr. Renata Veselská, Ph.D., M.Sc. (lecturer)
doc. RNDr. Jakub Neradil, Ph.D. (lecturer) - Guaranteed by
- prof. RNDr. Renata Veselská, Ph.D., M.Sc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Renata Veselská, Ph.D., M.Sc. - Timetable
- Tue 13:00–14:50 C03/117
- Prerequisites (in Czech)
- Bi3060 Basic genetics && Bi4020 Molecular biology
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Cellular and Molecular Diagnostics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, N-BI, specialization Genomika a proteomika)
- Molecular Biology and Genetics (programme PřF, N-BI, specialization Molekularni a geneticka diagnostika)
- General Biology (programme PřF, N-BI)
- General Biology (programme PřF, N-BI, specialization Physiology of Animals)
- Course objectives
- At the end of the course, students should be able to: understand in detail the specific aspects of the animal cell biology; explain the principles of main methodical approaches in animal cell biology; interpret the main application of the human cell biology in biomedicine (reproductive medicine, cell therapies).
- Syllabus
- 1) INTRODUCTION TO THE ANIMAL CELL BIOLOGY: Organization of the living systems, cellular and non-cellular systems, basic characteristics of the prokaryotic and eukaryotic cells, types of the eukaryotic cells, evolution of the cell.
- 2) IN VITRO STUDIES OF THE ANIMAL CELLS: In vitro cultivation of the animal cells, tissue cultures and cell cultures, organ cultures, characterization of the diploid and transformed cell lines, practical applications.
- 3) METHODICAL APPROACHES IN ANIMAL CELL BIOLOGY: Imaging methods, flow cytometry, metabolic assays, study of the living cells, micromanipulation techniques.
- 4) BIOMEMBRANES: Basic characteristics, compartmentalization - structural and functional aspects, membrane fusion, protoplasts, chimera creation, practical application - hybridoma technology.
- 5) CYTOSKELETON: Cytoskeletal components, asociated proteins, structural and functional aspects, intracellular transport, motor proteins, cell motility, muscle contraction, nuclear and membrane skeleton.
- 6) CELL CYCLE AND CELL REPRODUCTION: Phases of the cell cycle, cell cycle checkpoints, regulation of the cell cycle, mitosis, cytokinesis, extracellular control of the cell proliferation.
- 7) FERTILISATION AND EMBRYOGENESIS: Meiosis a gametogenesis, mechanisms of the fertilisation, embryogenesis, assisted reproduction, reproductive cloning.
- 8) CELL DIFFERENTIATION: Totipotence, pluripotence, multipotence, stem and progenitor cells, differentiation pathways, growth factors, induced differentiation, failures in the cell differentiation and their consequences.
- 9) STEM CELLS: Organization and renewal of the tissues, characteristics of the stem cells, adult and embryonic stem cells, cancer stem cells, stem cell lines in the in vitro conditions.
- 10) CELL THERAPIES: In vitro cultivation of the artifical organ and tissue derivatives, cell therapies, immunotherapies, differentiation therapies, use of the adult and embryonic stem cells, therapeutic cloning.
- 11) CELL PATHOLOGY: Reaction of the animal cell on the stress condicition, types of the stress factors, physical stress factors (mechanical damage, irradiation, heat shock and cold shock), toxines, specific inhibitors, biological stress factors, practical applications(radiobiology, cryobiology).
- 12) CELL DEATH: Features of the cell death, cytotoxic and cytostatic effect, programmmed and catastrophic cell death, necrosis and apoptosis, clinical aspects.
- Literature
- NEČAS, Oldřich, Augustin SVOBODA, Milan HEJTMÁNEK, Roman JANISCH, Miroslav ČERVINKA, Karel LENHART and Zdeněk KOLÁŘ. Obecná biologie pro lékařské fakulty (General Biology for Medical Faculties). 3. přepracované, v H+H 1. Jinočany: H+H, 2000, 554 pp. ISBN 80-86022-46-3. info
- ALBERTS, Bruce. Základy buněčné biologie :úvod do molekulární biologie buňky. 1. vyd. Ústí nad Labem: Espero, 1999, xxvi, 630. ISBN 80-902906-0-4. info
- Teaching methods
- Lectures.
- Assessment methods
- written test and oral exam
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Enrolment Statistics (Spring 2010, recent)
- Permalink: https://is.muni.cz/course/sci/spring2010/Bi8120