C5850 Biophysical Chemistry I

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Libuše Trnková, CSc. (lecturer)
Guaranteed by
prof. RNDr. Libuše Trnková, CSc.
Department of Chemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Chemistry - Chemistry Section - Faculty of Science
Timetable
Fri 8:00–9:50 C12/311
Prerequisites
Absolutorim from physical chemistry and biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
1. Strategy and tactics of biophysical chemistry (basic questions and strategic approaches of biophysical chemistry, structural features of biopolymers, main role of CO2 and water in biological systems)

2. Some problems concerning space structure of biopolymers (tertiary and quaternary structure of biopolymers, stability of helical structures, hydrogen and disulphidic bonds, hydrophobic interactions)

3. Interaction of biological molecules with electromagnetic radiation (absorption of electrons, fluorescence and infrared spectroscopies, optical activity, light scattering, resonance methods; structure and function of biopolymers)

4. Size and shape of macromolecules (electron microscopy, hydrodynamic methods, diffusion, sedimentation and centrifugation, Roentgen crystallography)

5. Thermodynamics of biological systems (1) (specificity of biological system, statistical interpretation of entropy, role of entropy in biological processes, production of entropy, problem of standard states; ideal, regular and nonregular solutions, multi-component systems, dissipative structures)

6. Thermodynamics of biological systems (2) (formalism of non-equilibrium thermodynamics, energetics of evolution and regeneration processes)

7. Behaviour of macromolecules in solution (statistical interpretation of polymer solutions, Huggins-Flory conception, dialyse equilibrium, osmotic pressure of the polymer component/Donnan, Scatchard/, formation of random coil)

8. Polyelectrolytes (PEL) (theory of PEL at limiting concentration, distribution of counterions, expansion of polyions, thermodynamics, transport and electrical behaviour of PEL, models of PEL)

9. Kinetics of biological systems (kinetic processes with several substrates and intermediates, catalysis, enzymatic reactions and regulation of them, biological clocks)

10. Polarography and voltammetry of biological molecules (use of polarographic and voltammetric techniques in research of electrochemical behaviour of proteins, nucleic acids and its components; analysis of farmaceutic important molecules)

11. Electron and ions transfer processes in biological systems.
Syllabus
  • Strategy and Tactics of Biophysical Chemistry.

    Some problems concerning space structure of biopolymers.

    Interaction of Biological Molecules with Electromagnetic Radiation.

    Size and Shape of Macromolecules.

    Thermodynamics of Biological Systems.

    Statistical Interpretation of Polymer Solution.

    Behaviour of Macromolecules in Solution.

    Polyelectrolytes.

    Kinetics of Biological Systems.

    Polarography and Voltammetry of Biological Molecules.

    Electron and Ion Transfer Processes in Biological System.
Literature
    required literature
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2., přeprac. a rozš. vyd. Praha: Academia, 2000. 337 s. ISBN 8020007911. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part II, Techniques for the study of biological structure and function. 12th print. New York: W.H. Freeman and Company, 2001. xxix, s. 3. ISBN 0-7167-1189-3. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part I, The conformation of biological macromolecules. 11th print. New York: W.H. Freeman and Company, 1999. xxii, 341. ISBN 0-7167-1042-0. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part III, The behavior of biological macromolecules. New York: W.H. Freeman and Company, 1980. xxix, s. 8. ISBN 0-7167-1191-5. info
    recommended literature
  • KALOUS, Vítěz and Zdeněk PAVLÍČEK. Biofyzikální chemie. 1. vyd. Praha: Nakladatelství technické literatury, 1980. 349 s. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992. 1237 s. ISBN 3-527-28242-4. info
  • H. Eisenberg: Biological Macromolecules and Polyelectrolytes in Solutions, Clarendon Press, Oxford 1976
  • MORAWETZ, Herbert. Chování makromolekul v roztoku : Macromolecules in solution (Orig.). Vyd. 1. Praha: Academia, nakladatelství Československé akademie věd, 1971. 512 s. info
Teaching methods
lectures, discussion, theoretical training, ppt presentation, solution of typical examples
Assessment methods
two written tests and oral examination
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 1999, Spring 2011 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020.
  • Enrolment Statistics (Spring 2014, recent)
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