C5850 Introduction to Biophysical Chemistry

Faculty of Science
Spring 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Libuše Trnková, CSc. (lecturer)
RNDr. Mgr. Jozef Hritz, Ph.D. (lecturer)
Mgr. Zdeněk Farka, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Libuše Trnková, CSc.
Department of Chemistry - Chemistry Section - Faculty of Science
Supplier department: Department of Chemistry - Chemistry Section - Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Mon 10:00–11:50 C12/311
Prerequisites
Absolutorim from physical chemistry and biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
1. Strategy and tactics of biophysical chemistry (basic questions and strategic approaches of biophysical chemistry, structural features of biopolymers, main role of CO2 and water in biological systems)

2. Some problems concerning space structure of biopolymers (tertiary and quaternary structure of biopolymers, stability of helical structures, hydrogen and disulphidic bonds, hydrophobic interactions)

3. Main determinates of biomolecular shape (Ramachandran plot, trans-cis preference of aminoacids, specific role of proline, hydrophobic interactions, distribution of aminoacids over protein structure)

4. Size and shape of macromolecules in solution (statistical interpretation of polymer solutions, dialysis, osmotic pressure, hydrodynamic methods, diffusion)

5. Canonical structural ensemble (Boltzmann probability, Gibbs and Helmholtz free energy, enthalpy, statistical interpretation of entropy)

6. Thermodynamics of biological systems (1) (stability of protein elements, folding and denaturation of biopolymers, first and second order phase transitions)

7. Thermodynamics of biological systems (2) (thermodynamics of biological processes, Molten-Globule state of proteins, formalism of non-equilibrium thermodynamics)

8. Polyelectrolytes (PEL) (theory of PEL at limiting concentration, distribution of counterions, expansion of polyions, thermodynamics, transport and electrical behavior of PEL, models of PEL)

9. Kinetics of biological systems (kinetic processes with several substrates and intermediates, catalysis, enzymatic reactions and regulation of them, biological clocks)

10. Polarography and voltammetry of biological molecules (use of polarographic and voltammetric techniques in research of electrochemical behaviour of proteins, nucleic acids and its components; analysis of pharmaceutical important molecules)

11. Electron and ions transfer processes in biological systems
Learning outcomes
Knowledge of physical chemistry will be enhanced by solving biological problems.
Syllabus
  • Strategy and Tactics of Biophysical Chemistry.

    Some problems concerning space structure of biopolymers.

    Interaction of Biological Molecules with Electromagnetic Radiation.

    Size and Shape of Macromolecules.

    Thermodynamics of Biological Systems.

    Statistical Interpretation of Polymer Solution.

    Behaviour of Macromolecules in Solution.

    Polyelectrolytes.

    Kinetics of Biological Systems.

    Polarography and Voltammetry of Biological Molecules.

    Electron and Ion Transfer Processes in Biological System.
Literature
    required literature
  • KODÍČEK, Milan and Vladimír KARPENKO. Biofysikální chemie. 2., přeprac. a rozš. vyd. Praha: Academia, 2000. 337 s. ISBN 8020007911. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part II, Techniques for the study of biological structure and function. 12th print. New York: W.H. Freeman and Company, 2001. xxix, s. 3. ISBN 0-7167-1189-3. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part I, The conformation of biological macromolecules. 11th print. New York: W.H. Freeman and Company, 1999. xxii, 341. ISBN 0-7167-1042-0. info
  • CANTOR, Charles R. and Paul R. SCHIMMEL. Biophysical chemistry. Part III, The behavior of biological macromolecules. New York: W.H. Freeman and Company, 1980. xxix, s. 8. ISBN 0-7167-1191-5. info
    recommended literature
  • KALOUS, Vítěz and Zdeněk PAVLÍČEK. Biofyzikální chemie. 1. vyd. Praha: Nakladatelství technické literatury, 1980. 349 s. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992. 1237 s. ISBN 3-527-28242-4. info
  • H. Eisenberg: Biological Macromolecules and Polyelectrolytes in Solutions, Clarendon Press, Oxford 1976
  • MORAWETZ, Herbert. Chování makromolekul v roztoku : Macromolecules in solution (Orig.). Vyd. 1. Praha: Academia, nakladatelství Československé akademie věd, 1971. 512 s. info
    not specified
  • FINKEL‘ŠTEJN, Aleksej Vital‘jevič and Oleg Borisovič PTICYN. Protein physics : a course of lectures. Amsterdam: Academic Press, 2002. xix, 354. ISBN 0122567811. info
Teaching methods
lectures, discussion, theoretical training, ppt presentation, solution of typical examples
Assessment methods
two written tests and oral examination
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Autumn 1999, Spring 2011 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020.
  • Enrolment Statistics (Spring 2017, recent)
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