VLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2023
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Taught in person.
doc. Mgr. Zdenka Fohlerová, Ph.D. (seminar tutor)
Mgr. David Galuška (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Pavel Šmak (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
Mgr. Patrícia Kittová (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
PharmDr. Kristýna Šebrlová, Ph.D. (assistant), PharmDr. Kristýna Šebrlová, Ph.D. (deputy)
Bc. Ivana Ševčíková (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
VLBC0422c/01: Mon 13. 2. to Thu 4. 5. Thu 14:00–16:30 A16/211; and Thu 18. 5. 14:00–16:30 A16/211, Thu 25. 5. 14:00–16:30 A16/211, J. Gregorová
VLBC0422c/02: Mon 13. 2. to Thu 4. 5. Thu 14:00–16:30 A16/216; and Thu 18. 5. 14:00–16:30 A16/216, Thu 25. 5. 14:00–16:30 A16/216, M. Králíková
VLBC0422c/03: Tue 14:00–16:30 A16/211, J. Kos
VLBC0422c/04: Mon 8:00–10:30 A16/216, J. Smutná
VLBC0422c/05: Mon 8:00–10:30 A16/211, Z. Fohlerová
VLBC0422c/06: Mon 8:00–10:30 A16/212, J. Gregorová
VLBC0422c/07: Mon 13. 2. to Thu 4. 5. Thu 14:00–16:30 A16/212; and Thu 18. 5. 14:00–16:30 A16/212, Thu 25. 5. 14:00–16:30 A16/212, O. Peš
VLBC0422c/08: Tue 14:00–16:30 A16/212, P. Šmak
VLBC0422c/09: Tue 14:00–16:30 A16/216, D. Galuška
VLBC0422c/10: Fri 8:00–10:30 A16/212, J. Víteček
VLBC0422c/11: Fri 8:00–10:30 A16/211, D. Galuška
VLBC0422c/12: Fri 8:00–10:30 A16/216, J. Kos
VLBC0422c/13: Mon 11:00–13:30 A16/211, J. Smutná
VLBC0422c/14: Mon 11:00–13:30 A16/212, Z. Fohlerová
VLBC0422c/15: Mon 11:00–13:30 A16/216, J. Gregorová
VLBC0422c/16: Mon 14:00–16:30 A16/216, J. Kos
VLBC0422c/17: Mon 14:00–16:30 A16/211, J. Gregorová
VLBC0422c/18: Mon 14:00–16:30 A16/212, O. Peš
VLBC0422c/19: Fri 11:00–13:30 A16/212, J. Víteček
VLBC0422c/20: Fri 11:00–13:30 A16/211, J. Smutná
Prerequisites (in Czech)
VLBC0321s Biochemistry I - seminar && VLFY0321c Physiology I - practice && VLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to VLBC0421p. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
After completing the course, the student will be able to:
- explain the methods of obtaining biological material for biochemical analyzes and know the rules of handling.
- describe the physiological values of the most important biochemical parameters.
- interpret the results of biochemical analyzes, discuss the significance of the findings.
- understand the principles of the most important biochemical-analytical methodologies.
- be oriented in basic laboratory tasks.
  • Introduction to practice - organization, work and safety principles, principles of pipetting
  • Selected examinations of glucose metabolism - P/B/U-glucose, glycemic profile, OGTT, U-ketone bodies
  • Selected examinations of lipid and cholesterol metabolism - S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol
  • Selected liver tests - ALT/AST, S/U-bilirubin, U-UBG
  • Investigation of proteins in blood serum - S-total protein, S-albumin, electrophoresis, S/B-CRP, S-ferritin
  • Selected kidney examinations - S/U-creatinine, creatinine clearance, eGFR, FR and FE water, U-proteins, U-albumin, S/U-urea, nitrogen balance
  • Chemical qualitative analysis of urine - diagnostic strips
  • Selected examinations of urolithiasis - S/U-uric acid, stones
    required literature
  • Studijní podklady a materiály k předmětu dostupné v IS MUNI.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes, missed exercises must be made up,
  • elaboration and approval of all protocols.
    The detailed conditions are posted in IS MUNI (Study materials – Course-related instructions).
  • Language of instruction
    Further comments (probably available only in Czech)
    The course is taught annually.
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu VLBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.
    A white coat, slippers or sturdy shoes with overshoes, gloves, a calculator are required.
    Consultations are possible after a personal arrangement with the teacher.
    The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2024.
    • Enrolment Statistics (spring 2023, recent)
    • Permalink: https://is.muni.cz/course/med/spring2023/VLBC0422c