VLBC0422c Biochemistry II

Faculty of Medicine
Spring 2018
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Ing. Martina Čarnecká, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
PharmDr. Katarína Kostolanská (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
Mgr. Lucie Novotná (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Anna Pleskačová, Ph.D. (seminar tutor)
Mgr. Roman Sándor (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
MUDr. Lenka Gescheidtová (assistant)
Lenka Nerudová (assistant)
MUDr. RNDr. Michal Řiháček (assistant)
Mgr. Ondřej Wiewiorka (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Supplier department: Department of Biochemistry - Theoretical Departments - Faculty of Medicine
Timetable of Seminar Groups
VLBC0422c/01: Mon 10:15–12:45 A16/211, M. Tomandlová
VLBC0422c/02: Mon 10:15–12:45 A16/211, M. Hlaváčová
VLBC0422c/03: Mon 10:15–12:45 A16/211, J. Gregorová
VLBC0422c/04: Mon 7:30–10:00 A16/211, M. Čarnecká
VLBC0422c/05: Mon 7:30–10:00 A16/211, M. Hlaváčová
VLBC0422c/06: Mon 7:30–10:00 A16/211, J. Gregorová
VLBC0422c/07: Wed 10:45–13:15 A16/211, J. Gregorová
VLBC0422c/08: Tue 13:00–15:30 A16/211
VLBC0422c/09: Tue 13:00–15:30 A16/211
VLBC0422c/10: Tue 13:00–15:30 A16/211
VLBC0422c/11: Wed 10:45–13:15 A16/211, M. Hlaváčová
VLBC0422c/12: Wed 10:45–13:15 A16/211, M. Králíková
VLBC0422c/13: Thu 14:30–17:00 A16/211, M. Čarnecká
VLBC0422c/14: Thu 14:30–17:00 A16/211
VLBC0422c/15: Thu 14:30–17:00 A16/211
VLBC0422c/16: Mon 13:30–16:00 A16/211, M. Čarnecká
VLBC0422c/17: Mon 13:30–16:00 A16/211, M. Hlaváčová
VLBC0422c/18: Mon 13:30–16:00 A16/211, M. Tomandlová
Prerequisites (in Czech)
( VLBI0222p Biology II-lect. || VSBI0222p Biology II-lect. ) && VLBF011p Biophysics-lect. && VLBC0321s Biochemistry I sem && VLBC0321c Biochemistry I && VLFY0321c Physiology I - practice && VLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to VLBC0421p a VLLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
The student will explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation. Describes the physiological values of the most important biochemical parameters. Interprets the results of biochemical analyzes, discusses the significance of the findings. Understands the principles of the most important biochemical-analytical methodologies. Is oriented in basic laboratory manipulations.
  • • Directions for laboratory work. Handling biological material. Laboratory examination. Blood, plasma, serum. Blood collection. Blood processing. Manual methods, pipetting. Biochemical analyzers. Test requisition forms. • Investigations of glucose metabolism. Diabetes mellitus, Enzymatic determination of plasma glucose. Determination of glycaemia by personal glucometer. Oral glucose tolerance test (oGTT). Detection and determination of glucose in urine. Detection of ketone bodies in urine. Determination of glycated hemoglobin (HbA1c). • Investigation of lipid and cholesterol metabolism. Enzymatic determination of serum total cholesterol. Determination of blood cholesterol using Accutrend. Determination of blood triacylglycerols using Accutrend. Determination of HDL-cholesterol and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. • Investigations in liver disease I – Enzymes. Determination of catalytic concentrations of aminotranferases in serum. Determination of ALP catalytic concentration in serum. • Selected investigations in liver disease II – Bile pigments and albumin. Determination of total bilirubin in serum. Detection of bilirubin in urine. Detection of urobilinogens in urine. Determination of serum albumin. • Investigation of plasma proteins. Determination of total protein by the biuret test. Determination of albumin in serum. Electrophoresis of serum proteins. Assay for increased level of C-reactive protein. Determination of serum IgE by ELISA method. • Biochemical tests of renal function – I. Investigation of urine by inspection and physical methods. Determination of creatinine in serum and urine. Estimation of glomerular filtration rate from creatinine clearance. Fractional reabsorption and excretion of water. • Biochemical tests of renal function – II. Detection of proteinuria. Urine test for haem compounds. Determination of protein concentration in urine. Detection of microalbuminuria. Detection of Bence Jones protein in urine. Determination of urea in serum and urine. Approximate assessment of the nitrogen balance. • Routine urinalysis. Test strips. Multipurpose diagnostic strips in urinalysis. Detection of addictive drugs in urine. Detection of barbiturates in urine. Detection of luteinizing hormone in urine. • Examinations in urolithiasis. Solubility of uric acid. Detection of uric acid by murexide reaction. Simplified chemical analysis of renal calculi. Determination of uric acid in serum and urine. • Some investigations of the gastrointestinal tract. Selected examinations of stomach functions. Laboratory tests in pancreas diseases. Test of occult bleeding in gastrointestinal tract.
    required literature
  • Instructions for practicals available in Information system
  • Podklady ke cvičení dostupné v Informačním systému
Teaching methods
laboratory practicals
Assessment methods
Practicals in biochemistry lab.
Full attendance in all lessons (making up all missing and justified lessons. Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry I.
Language of instruction
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
The course is also listed under the following terms Spring 2017, spring 2019, spring 2020, spring 2021.
  • Enrolment Statistics (Spring 2018, recent)
  • Permalink: https://is.muni.cz/course/med/spring2018/VLBC0422c