VLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2027
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Mgr. David Galuška, Ph.D. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
Ing. Bc. Hana Logerová, Ph.D. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Iva Audy (assistant)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Michaela Šimurdová (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
VLBC0321s Biochemistry I - seminar && VLFY0321c Physiology I - practice && VLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Abstract
This practical course complements the lecture-based course Biochemistry II. Its objective is to provide students with hands-on experience in solving practical problems in clinical biochemistry, to develop essential laboratory skills, and to learn how to interpret the results of clinical biochemical analyses. Students become familiar with major biochemical-analytical methods, physiological ranges of key biochemical parameters, and their diagnostic significance in various pathological conditions.
Learning outcomes
Upon successful completion of the course, the student will be able to:
  • explain the methods of obtaining biological material for biochemical analyses and understand the principles of correct handling;
  • describe the physiological reference values of essential biochemical parameters;
  • interpret the results of biochemical analyses and discuss their clinical significance;
  • understand the principles of important biochemical‑analytical methodologies;
  • perform and navigate basic laboratory techniques and safety procedures.
Key topics
    • Introduction to practical classes: laboratory safety, rules of conduct, pipetting principles.
    • Selected tests of glucose metabolism: plasma/blood/urine glucose, glycaemic profile, OGTT, urinary ketone bodies.
    • Selected tests of lipid and cholesterol metabolism: serum/blood cholesterol, serum triglycerides, LDL/HDL/non‑HDL cholesterol.
    • Selected liver function tests: serum ALT/AST, serum/urine bilirubin, urinary urobilinogen.
    • Selected serum protein tests: total protein, albumin, electrophoresis, CRP, ferritin.
    • Selected renal function tests: serum/urine creatinine, creatinine clearance, eGFR, fractional reabsorption and excretion of water, urinary proteins and albumin, serum/urine urea, nitrogen balance.
    • Chemical qualitative urine analysis: diagnostic dipsticks.
    • Selected examinations relevant to urolithiasis: serum/urine uric acid, urinary calculi.
Study resources and literature
    required literature
  • Interaktivní osnova v IS MU.
Approaches, practices, and methods used in teaching
Laboratory-based instruction focused on the practical performance of selected biochemical analyses and discussion of the diagnostic significance of individual tests.
Method of verifying learning outcomes and course completion requirements
Course-unit credit. Conditions for giving the course-unit credit:
1) completion of all classes, 2) elaboration and approval of all protocols.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu VLBC0422p.
Listed among pre-requisites of other courses
Teacher's information

Detailed information regarding the organisation of teaching and course completion requirements is available in the Study Materials section of IS MU.

Attendance requires a laboratory coat, indoor footwear or protective overshoes, gloves, and a calculator.

Consultations are available upon personal arrangement with the teacher.

The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025, spring 2026.
  • Enrolment Statistics (spring 2027, recent)
  • Permalink: https://is.muni.cz/course/med/spring2027/VLBC0422c