BLMB011c Molecular and Cellular Biology - practice

Faculty of Medicine
autumn 2019
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Kateřina Cetkovská, Ph.D. (lecturer)
prof. RNDr. Ondřej Slabý, Ph.D. (lecturer)
Mgr. Kateřina Vopěnková, Ph.D. (lecturer)
Ing. Lívia Eiselleová, Ph.D. (seminar tutor)
Mgr. Bohumil Fafílek, Ph.D. (seminar tutor)
Mgr. Petr Fojtík (seminar tutor)
Mgr. Matěj Hrala (seminar tutor)
Mgr. Petra Lázničková (seminar tutor)
Mgr. Markéta Nováková, Ph.D. (seminar tutor)
Mgr. Veronika Palušová, Ph.D. (seminar tutor)
MUDr. Martin Pešl, Ph.D. (seminar tutor)
Mgr. Petra Pospíšilová, Ph.D. (seminar tutor)
Mgr. Vladimír Rotrekl, Ph.D. (seminar tutor)
doc. MUDr. Iva Slaninová, Ph.D. (seminar tutor)
Ing. Natália Vadovičová (seminar tutor)
Mgr. Barbora Valčíková (seminar tutor)
Mgr. Kateřina Vopěnková, Ph.D. (seminar tutor)
RNDr. Eliška Vrbová (seminar tutor)
Jana Růžičková (assistant)
Guaranteed by
prof. Ing. Petr Dvořák, CSc.
Department of Biology - Theoretical Departments - Faculty of Medicine
Contact Person: Ing. Lívia Eiselleová, Ph.D.
Supplier department: Department of Biology - Theoretical Departments - Faculty of Medicine
Timetable
Wed 7:30–8:30 B07/205a, Wed 8:30–9:30 B07/217, Wed 8:30–9:30 B07/205a, Wed 9:30–10:00 B07/205a
Prerequisites
Knowledge of high-school biology on the level of Overview of High School Biology (https://is.muni.cz/auth/do/med/el/overview/overview_CZ.qwarp) or similar is required.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are:
- learning to operate the light microscope while observing prokaryotic and eukaryotic cells and various cell structures and processes
- understanding the methods of risk evaluation for monogenic hereditary diseases with autosomal inheritance in patients based on family anamneses
Learning outcomes
After completing the course, the student will be able to:
- operate the light microscope
- perform some of the basic cytochemical staining techniques in observation of bacterial and human cells
- explain the basic principles and use of electron microscopy
- describe the basic techniques and applications of tissue and cell cultures
- deduce the impact of selected DNA mutations on the primary structure of proteins
- explain the methodology of assembling and examining the karyotype and its use, and to evaluate basic numerical and structural aberrations in assigned human karyotypes
- evaluate the risk of numerical and structural aberrations resulting from meiotic non-disjunctions and chromosomal translocations
- evaluate the risk of monogenic hereditary diseases with autosomal inheritance in patients based on assigned family anamneses of the diseases
Syllabus
  • Week 1: Instructions. Non-cellular organisms and infectious agents
  • Week 2: Prokaryotic cells and their medical significance
  • Week 3: Microscopic observation of eukaryotic cells
  • Week 4: In vitro culture of human cells
  • Week 5: DNA structure and replication. Transcription and translation
  • Week 6: Principles of electron microscopy, cellular ultrastructure
  • Week 7: Plasma membrane - osmosis, cell fusion
  • Week 8: Methods of cell cycle studying
  • Week 9: Mitosis observation in a light microscope
  • Week 10: Biological significance of meiosis - gametogenesis
  • Week 11: Human karyotype and chromosomal abnormalities
  • Week 12: Genetic disorders - autosomal inheritance
  • Week 13: substitution week
Literature
    required literature
  • Aktuální protokoly ke cvičením jsou dostupné v elektronické podobě na stránkách informačního systému MU (ISu): Studijní materiály předmětu BLMB011c
Teaching methods
laboratory practice, class discussion
Assessment methods
Practical classes are mandatory.
Requirements for the course completion: full attendance in the practices (1 absence, excused or unexcused, is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; activity in the practices.
In case of student’s late arrival to the practice or poor activity at the practice, the student is required to write an essay in the length of 2 pages on a given topic. Same for the case of 2nd and any additional absence in practices (excused or unexcused). Find the instructions for essay writing in the Course-related instructions in the IS, bring the essay printed to the teacher who specified the topic; be ready to answer the teacher's questions regarding the topic.
In case of 3 absences, (or more than 3 absences all excused by the Office for Studies within 5 days from the beginning of the absence and introduced to the Information System), it is addressed individually and course-unit credit is awarded only after the proof of the student's knowledge of the entire semester topics from lectures and practices in a Credit test (20 questions: 10 test questions, multiple answers can be correct, negative marking is obtained for incorrect answers + 10 given terms for written explanation. At least 10 points out of 20 are needed to pass.)
In the case of 4 or more unexcused absences, the course-unit credit is not awarded.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
Teacher's information
https://is.muni.cz/auth/el/1411/podzim2019/BLMB011c/index.qwarp
What to bring to each practice: printed protocol for the respective practice, your own writing materials. Download the protocol for each practice from the Study materials in the IS, and study it in advance. Lab shoes, lab coats and disposable gloves are not required, but for the tasks with staining, lab coats are recommended.

Complete Biology instructions to be found at the following link: https://is.muni.cz/auth/el/1411/podzim2018/BLMB011c/op/Pokyny_ke_cvicenim_ZDRL_denni.pdf

The course is also listed under the following terms Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2020.
  • Enrolment Statistics (autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2019/BLMB011c