VLBC0422c Biochemistry II - practice

Faculty of Medicine
spring 2025
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
PharmDr. Kristýna Šebrlová, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Martina Hanousková (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
prof. RNDr. Eva Táborská, CSc. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
VLBC0321s Biochemistry I - seminar && VLFY0321c Physiology I - practice && VLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to VLBC0421p. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
After completing the course, the student will be able to:
- explain the methods of obtaining biological material for biochemical analyzes and know the rules of handling.
- describe the physiological values of the most important biochemical parameters.
- interpret the results of biochemical analyzes, discuss the significance of the findings.
- understand the principles of the most important biochemical-analytical methodologies.
- be oriented in basic laboratory tasks.
Syllabus
  • Introduction to practice - organization, work and safety principles, principles of pipetting
  • Selected examinations of glucose metabolism - P/B/U-glucose, glycemic profile, OGTT, U-ketone bodies
  • Selected examinations of lipid and cholesterol metabolism - S/B-cholesterol, S-TG, S-LDL/HDL/non-HDL-cholesterol
  • Selected liver tests - ALT/AST, S/U-bilirubin, U-UBG
  • Investigation of proteins in blood serum - S-total protein, S-albumin, electrophoresis, S/B-CRP, S-ferritin
  • Selected kidney examinations - S/U-creatinine, creatinine clearance, eGFR, FR and FE water, U-proteins, U-albumin, S/U-urea, nitrogen balance
  • Chemical qualitative analysis of urine - diagnostic strips
  • Selected examinations of urolithiasis - S/U-uric acid, stones
Literature
    required literature
  • Studijní podklady a materiály k předmětu dostupné v IS MU.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all classes, missed exercises must be made up,
  • elaboration and approval of all protocols.
    Detailed conditions are posted in IS MU.
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    The course is taught: every week.
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu VLBC0422p.
    Information on the extent and intensity of the course: 45.
    Listed among pre-requisites of other courses
    Teacher's information
    A white coat, slippers or sturdy shoes with overshoes, gloves, a calculator are required.
    Consultations are possible after a personal arrangement with the teacher.
    The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024.
    • Enrolment Statistics (spring 2025, recent)
    • Permalink: https://is.muni.cz/course/med/spring2025/VLBC0422c